Restriction map for
pF1KE0295
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tsp509I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 675 GTAC GT^AC
Alw44I 580 GTGCAC G^TGCAC
ApaLI 580 GTGCAC G^TGCAC
AspI 421 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 31 GTCTTC GAAGAC(2/6)
BcgI 138 CGAGTCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 151 CTAG C^TAG
BpuAI 31 GTCTTC GAAGAC(2/6)
BsaI 22 GAGACC GGTCTC(1/5)
BseRI 111 CTCCTC GAGGAG(10/8)
BsgI 604 CTGCAC GTGCAG(16/14)
BsmAI 22 GAGAC GTCTC(1/5)
BsrBI 108 CCGCTC CCGCTC(-3/-3)
BstAPI 582 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 180 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 523 CTCTTC CTCTTC(1/4)
EcoT14I 215 CCAAGG C^C[AT][AT]GG
HgaI 297 GACGC GACGC(5/10)
HinfI 139 GAGTC G^A[ACGT]TC
HphI 230 TCACC GGTGA(8/7)
Ksp632I 523 CTCTTC CTCTTC(1/4)
MaeI 151 CTAG C^TAG
MaeII 578 ACGT A^CGT
MaeIII 506 GTTAC ^GT[ACGT]AC
NaeI 444 GCCGGC GCC^GGC
NgoMIV 444 GCCGGC G^CCGGC
NspI 188 GCATGT [GA]CATG^[CT]
PflFI 421 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 139 GAGTC GAGTC(4/5)
PpuMI 164 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 24 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 301 CTGCAG CTGCA^G
RsaI 675 GTAC GT^AC
SfaNI 260 GATGC GCATC(5/9)
SfcI 301 CTGCAG C^T[GA][CT]AG
StuI 305 AGGCCT AGG^CCT
StyI 215 CCAAGG C^C[AT][AT]GG
TaqI 665 TCGA T^CGA
Tth111I 421 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 665 TCGA T^CGA
XcmI 332 CCATCTGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 283 GGCGCC G[GA]^CG[CT]C
395 GGCGCC
AspHI 130 GAGCAC G[AT]GC[AT]^C
580 GTGCAC
BbeI 283 GGCGCC GGCGC^C
395 GGCGCC
BpmI 118 CTGGAG CTGGAG(16/14)
700 CTGGAG
BsaHI 283 GGCGCC G[GA]^CG[CT]C
395 GGCGCC
BsiHKAI 130 GAGCAC G[AT]GC[AT]^C
580 GTGCAC
BsmFI 419 GGGAC GGGAC(10/14)
90 GTCCC
BsrFI 232 ACCGGC [GA]^CCGG[CT]
444 GCCGGC
BsrI 52 ACTGG ACTGG(1/-1)
62 CCAGT
Cfr10I 232 ACCGGC [GA]^CCGG[CT]
444 GCCGGC
DpnII 228 GATC ^GATC
597 GATC
FauI 407 CCCGC CCCGC(4/6)
453 GCGGG
Hin1I 283 GGCGCC G[GA]^CG[CT]C
395 GGCGCC
KasI 283 GGCGCC G^GCGCC
395 GGCGCC
MboI 228 GATC ^GATC
597 GATC
NarI 283 GGCGCC GG^CGCC
395 GGCGCC
NdeII 228 GATC ^GATC
597 GATC
NlaIII 189 CATG CATG^
672 CATG
Sau3AI 228 GATC ^GATC
597 GATC
SfoI 283 GGCGCC GGC^GCC
395 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsoBI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EcoO109I EcoRII Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
SmaI TseI TspRI XmaCI XmaI