Restriction map for
pF1KE0281
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
HphI KpnI MaeI MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
Sau3AI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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BsaWI 314 TCCGGT [AT]^CCGG[AT]
Bsp1407I 338 TGTACA T^GTACA
BsrFI 629 ACCGGC [GA]^CCGG[CT]
BsrGI 338 TGTACA T^GTACA
BstAPI 311 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 629 ACCGGC [GA]^CCGG[CT]
DraIII 37 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 160 CTCTTC CTCTTC(1/4)
HincII 253 GTCAAC GT[CT]^[GA]AC
HindII 253 GTCAAC GT[CT]^[GA]AC
HinfI 556 GAGTC G^A[ACGT]TC
Ksp632I 160 CTCTTC CTCTTC(1/4)
KspI 240 CCGCGG CCGC^GG
MaeII 671 ACGT A^CGT
MslI 395 CACGGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 240 CCGCGG C[AC]G^C[GT]G
NspI 47 ACATGC [GA]CATG^[CT]
PflMI 36 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 556 GAGTC GAGTC(4/5)
PpuMI 423 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 694 CTGCAG CTGCA^G
SacII 240 CCGCGG CCGC^GG
SapI 159 GCTCTTC GCTCTTC(1/4)
SfcI 694 CTGCAG C^T[GA][CT]AG
SspBI 338 TGTACA T^GTACA
TspRI 84 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 36 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 241 CGCG CG^CG
680 CGCG
AcyI 6 GGCGCC G[GA]^CG[CT]C
632 GGCGCC
AluI 289 AGCT AG^CT
653 AGCT
BanI 6 GGCGCC G^G[CT][GA]CC
632 GGCGCC
BanII 441 GAGCCC G[GA]GC[CT]^C
600 GAGCCC
BbeI 6 GGCGCC GGCGC^C
632 GGCGCC
BpmI 438 CTGGAG CTGGAG(16/14)
487 CTGGAG
BsaHI 6 GGCGCC G[GA]^CG[CT]C
632 GGCGCC
BstUI 241 CGCG CG^CG
680 CGCG
DdeI 123 CTCAG C^T[ACGT]AG
554 CTGAG
DsaI 41 CCGTGG C^C[GA][CT]GG
240 CCGCGG
FauI 366 CCCGC CCCGC(4/6)
3 GCGGG
HaeII 6 GGCGCC [GA]GCGC^[CT]
632 GGCGCC
Hin1I 6 GGCGCC G[GA]^CG[CT]C
632 GGCGCC
KasI 6 GGCGCC G^GCGCC
632 GGCGCC
MvnI 241 CGCG CG^CG
680 CGCG
NarI 6 GGCGCC GG^CGCC
632 GGCGCC
NlaIII 48 CATG CATG^
523 CATG
SfoI 6 GGCGCC GGC^GCC
632 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AvaII BbvI BcnI
BglI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MspI MvaI MwoI NciI
NlaIV RsaI Sau96I ScrFI SfaNI
TseI