##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DdeI        
DpnII       DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     FauI        FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
MwoI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI           67  GAATTC               [GA]^AATT[CT]
AgeI          189  ACCGGT               A^CCGGT
AluI            8  AGCT                 AG^CT
ApoI           67  GAATTC               [GA]^AATT[CT]
AvaII         192  GGTCC                G^G[AT]CC
BbvI            9  GCTGC                GCAGC(8/12)
BcnI          144  CCCGG                CC^[GC]GG
BmyI          141  GTGCCC               G[AGT]GC[ACT]^C
BsaWI         189  ACCGGT               [AT]^CCGG[AT]
BsgI          111  CTGCAC               GTGCAG(16/14)
BsmAI         164  GTCTC                GTCTC(1/5)
Bsp1286I      141  GTGCCC               G[AGT]GC[ACT]^C
BsrFI         189  ACCGGT               [GA]^CCGG[CT]
BstF5I        147  GGATG                GGATG(2/0)
BstNI          33  CCTGG                CC^[AT]GG
Cac8I          97  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr10I        189  ACCGGT               [GA]^CCGG[CT]
DraII          94  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I       94  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI          67  GAATTC               G^AATTC
EcoRII         33  CCTGG                ^CC[AT]GG
EcoT22I       173  ATGCAT               ATGCA^T
Fnu4HI          9  GCTGC                GC^[ACGT]GC
FokI          147  GGATG                GGATG(9/13)
HaeIII         96  GGCC                 GG^CC
HgaI          151  GCGTC                GACGC(5/10)
ItaI            9  GCTGC                GC^[ACGT]GC
MnlI          184  CCTC                 CCTC(7/6)
MslI          202  CATGAATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I          7  CAGCTG               C[AC]G^C[GT]G
MvaI           33  CCTGG                CC^[AT]GG
NciI          144  CCCGG                CC^[GC]GG
NsiI          173  ATGCAT               ATGCA^T
NspI          131  ACATGC               [GA]CATG^[CT]
PinAI         189  ACCGGT               A^CCGGT
PvuII           7  CAGCTG               CAG^CTG
SfaNI         148  GATGC                GCATC(5/9)
TseI            9  GCTGC                G^C[AT]GC
Tsp509I        68  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BsiYI          33  CCTGGTTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              138  CCTGTGCCCGG         
BslI           33  CCTGGTTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              138  CCTGTGCCCGG         
BsmFI         153  GTCCC                GGGAC(10/14)
              193  GTCCC               
BssKI          33  CCTGG                ^CC[ACGT]GG
              144  CCCGG               
Cfr13I         95  GGGCC                G^G[ACGT]CC
              192  GGTCC               
HapII         145  CCGG                 C^CGG
              190  CCGG                
HpaII         145  CCGG                 C^CGG
              190  CCGG                
MspI          145  CCGG                 C^CGG
              190  CCGG                
NlaIV          94  GGGGCC               GG[ACGT]^[ACGT]CC
              192  GGTCCC              
Sau96I         95  GGGCC                G^G[ACGT]CC
              192  GGTCC               
ScrFI          33  CCTGG                CC^[ACGT]GG
              144  CCCGG               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

NlaIII