Restriction map for
pF1KE0279
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AvrII BamHI BanII BbeI BbvCI
BbvI BcgI BciVI BclI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI Fnu4HI FseI Hin1I HindIII
HpaI ItaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tru9I TseI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccII 334 CGCG CG^CG
AccIII 537 TCCGGA T^CCGGA
AcsI 217 GAATTC [GA]^AATT[CT]
AfaI 453 GTAC GT^AC
AhdI 343 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 363 GATCC GGATC(4/5)
ApoI 217 GAATTC [GA]^AATT[CT]
AspEI 343 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 301 GAGCAC G[AT]GC[AT]^C
AviII 245 TGCGCA TGC^GCA
BalI 371 TGGCCA TGG^CCA
BanI 595 GGCACC G^G[CT][GA]CC
BbrPI 304 CACGTG CAC^GTG
BbsI 139 GAAGAC GAAGAC(2/6)
BcnI 585 CCGGG CC^[GC]GG
BglII 107 AGATCT A^GATCT
BpuAI 139 GAAGAC GAAGAC(2/6)
BsaAI 304 CACGTG [CT]AC^GT[GA]
BsaI 581 GAGACC GGTCTC(1/5)
BsaWI 537 TCCGGA [AT]^CCGG[AT]
BseAI 537 TCCGGA T^CCGGA
BsiHKAI 301 GAGCAC G[AT]GC[AT]^C
BsiYI 569 CCAAATGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 569 CCAAATGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 581 GAGAC GTCTC(1/5)
BsmFI 16 GTCCC GGGAC(10/14)
BspEI 537 TCCGGA T^CCGGA
BsrDI 609 GCAATG GCAATG(2/0)
BssSI 367 CTCGTG CACGAG(-5/-1)
BstAPI 237 GCAATACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 410 CCAGG CC^[AT]GG
BstUI 334 CGCG CG^CG
BstYI 107 AGATCT [GA]^GATC[CT]
Cac8I 292 GCCAGC GC[ACGT]^[ACGT]GC
DraII 473 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 371 TGGCCA [CT]^GGCC[GA]
Eam1105I 343 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 473 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 217 GAATTC G^AATTC
EcoRII 410 CCAGG ^CC[AT]GG
EcoT14I 470 CCAAGG C^C[AT][AT]GG
FspI 245 TGCGCA TGC^GCA
HgaI 335 GCGTC GACGC(5/10)
HincII 490 GTCGAC GT[CT]^[GA]AC
HindII 490 GTCGAC GT[CT]^[GA]AC
MflI 107 AGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MroI 537 TCCGGA T^CCGGA
MscI 371 TGGCCA TGG^CCA
MspA1I 203 CAGCTG C[AC]G^C[GT]G
MvaI 410 CCAGG CC^[AT]GG
MvnI 334 CGCG CG^CG
NciI 585 CCGGG CC^[GC]GG
NspI 242 ACATGC [GA]CATG^[CT]
PmaCI 304 CACGTG CAC^GTG
PmlI 304 CACGTG CAC^GTG
PpuMI 473 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 203 CAGCTG CAG^CTG
RsaI 453 GTAC GT^AC
SalI 490 GTCGAC G^TCGAC
SfaNI 390 GATGC GCATC(5/9)
SmlI 396 CTTGAG C^T[CT][GA]AG
SspI 445 AATATT AAT^ATT
StyI 470 CCAAGG C^C[AT][AT]GG
TfiI 534 GATTC G^A[AT]TC
XcmI 35 CCACATACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 107 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 271 GTCTAC GT^[AC][GT]AC
490 GTCGAC
AvaII 309 GGACC G^G[AT]CC
474 GGACC
BmyI 80 GTGCCC G[AGT]GC[ACT]^C
301 GAGCAC
BsaJI 410 CCAGGG C^C[ACGT][ACGT]GG
470 CCAAGG
Bsp1286I 80 GTGCCC G[AGT]GC[ACT]^C
301 GAGCAC
BssKI 410 CCAGG ^CC[ACGT]GG
585 CCGGG
FauI 231 GCGGG CCCGC(4/6)
576 GCGGG
HaeII 10 AGCGCC [GA]GCGC^[CT]
223 AGCGCC
HapII 538 CCGG C^CGG
585 CCGG
HpaII 538 CCGG C^CGG
585 CCGG
HphI 86 TCACC GGTGA(8/7)
407 TCACC
MaeII 191 ACGT A^CGT
305 ACGT
MaeIII 23 GTGAC ^GT[ACGT]AC
280 GTCAC
MspI 538 CCGG C^CGG
585 CCGG
NlaIV 474 GGACCC GG[ACGT]^[ACGT]CC
595 GGCACC
PleI 142 GACTC GAGTC(4/5)
349 GAGTC
ScrFI 410 CCAGG CC^[ACGT]GG
585 CCGGG
TaqI 383 TCGA T^CGA
491 TCGA
Tsp45I 23 GTGAC ^GT[GC]AC
280 GTCAC
Tsp509I 218 AATT ^AATT
522 AATT
TthHB8I 383 TCGA T^CGA
491 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BsrI BstF5I CfoI
Cfr13I DpnII FokI HaeIII HhaI
HinP1I HinfI MboI MboII MnlI
MwoI NdeII NlaIII Sau3AI Sau96I
TspRI