Restriction map for
pF1KE0277
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmAI BsmBI BsmFI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 582 CCGC CCGC(-3/-1)
AflII 691 CTTAAG C^TTAAG
AgeI 389 ACCGGT A^CCGGT
AluI 304 AGCT AG^CT
Alw44I 808 GTGCAC G^TGCAC
ApaLI 808 GTGCAC G^TGCAC
AspHI 808 GTGCAC G[AT]GC[AT]^C
BamHI 275 GGATCC G^GATCC
BanII 460 GGGCTC G[GA]GC[CT]^C
BbvI 90 GCAGC GCAGC(8/12)
BfaI 38 CTAG C^TAG
BfrI 691 CTTAAG C^TTAAG
BlpI 584 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 584 GCTTAGC GC^T[ACGT]AGC
BsaWI 389 ACCGGT [AT]^CCGG[AT]
BsiHKAI 808 GTGCAC G[AT]GC[AT]^C
BsmI 932 GAATGC GAATGC(1/-1)
Bsp1407I 260 TGTACA T^GTACA
BspMI 745 ACCTGC ACCTGC(4/8)
BsrFI 389 ACCGGT [GA]^CCGG[CT]
BsrGI 260 TGTACA T^GTACA
CelII 584 GCTTAGC GC^T[ACGT]AGC
Cfr10I 389 ACCGGT [GA]^CCGG[CT]
EarI 721 GAAGAG CTCTTC(1/4)
EcoNI 564 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 51 GAATTC G^AATTC
Fnu4HI 90 GCAGC GC^[ACGT]GC
HapII 390 CCGG C^CGG
HpaII 390 CCGG C^CGG
ItaI 90 GCAGC GC^[ACGT]GC
Ksp632I 721 GAAGAG CTCTTC(1/4)
MaeI 38 CTAG C^TAG
MaeIII 614 GTTAC ^GT[ACGT]AC
MfeI 150 CAATTG C^AATTG
MspI 390 CCGG C^CGG
MunI 150 CAATTG C^AATTG
MwoI 183 GCCAAGCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 275 GGATCC GG[ACGT]^[ACGT]CC
PinAI 389 ACCGGT A^CCGGT
SfcI 900 CTACAG C^T[GA][CT]AG
SmlI 691 CTTAAG C^T[CT][GA]AG
SspBI 260 TGTACA T^GTACA
TaqI 294 TCGA T^CGA
TseI 90 GCAGC G^C[AT]GC
TthHB8I 294 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 51 GAATTC [GA]^AATT[CT]
517 AAATTT
AfaI 261 GTAC GT^AC
393 GTAC
ApoI 51 GAATTC [GA]^AATT[CT]
517 AAATTT
BmyI 460 GGGCTC G[AGT]GC[ACT]^C
808 GTGCAC
BseRI 120 CTCCTC GAGGAG(10/8)
370 CTCCTC
BsgI 190 CTGCAC GTGCAG(16/14)
565 CTGCAC
Bsp1286I 460 GGGCTC G[AGT]GC[ACT]^C
808 GTGCAC
BsrDI 318 CATTGC GCAATG(2/0)
396 CATTGC
BsrI 70 ACTGG ACTGG(1/-1)
324 CCAGT
BstYI 275 GGATCC [GA]^GATC[CT]
573 GGATCT
Cfr13I 225 GGCCC G^G[ACGT]CC
840 GGCCC
EcoT14I 134 CCATGG C^C[AT][AT]GG
339 CCATGG
MaeII 417 ACGT A^CGT
845 ACGT
MflI 275 GGATCC [GA]^GATC[CT]
573 GGATCT
MslI 757 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
801 CACAATGGTG
NcoI 134 CCATGG C^CATGG
339 CCATGG
PleI 941 GACTC GAGTC(4/5)
358 GAGTC
RsaI 261 GTAC GT^AC
393 GTAC
Sau96I 225 GGCCC G^G[ACGT]CC
840 GGCCC
StyI 134 CCATGG C^C[AT][AT]GG
339 CCATGG
XcmI 134 CCATGGTGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
843 CCACGTTCTCATTGG
XhoII 275 GGATCC [GA]^GATC[CT]
573 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AlwI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cac8I DdeI DpnII
DsaI EcoRII FokI HaeIII HinfI
MboI MboII MnlI MseI MvaI
NdeII NlaIII Sau3AI ScrFI SfaNI
TfiI Tru9I Tsp509I TspRI