##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BsrI        
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FokI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SalI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   20 enzymes) #
###################################

Aor51HI       297  AGCGCT               AGC^GCT
BbsI           18  GAAGAC               GAAGAC(2/6)
BpuAI          18  GAAGAC               GAAGAC(2/6)
BsgI          442  CTGCAC               GTGCAG(16/14)
BsiEI         379  CGACCG               CG[GA][CT]^CG
BspMI         240  GCAGGT               ACCTGC(4/8)
BsrBI         190  GAGCGG               CCGCTC(-3/-3)
BssHII        258  GCGCGC               G^CGCGC
BstAPI        425  GCAAGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         222  CCAGG                CC^[AT]GG
Eco47III      297  AGCGCT               AGC^GCT
EcoNI         342  CCTCCCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        222  CCAGG                ^CC[AT]GG
HgaI           64  GACGC                GACGC(5/10)
HphI          483  TCACC                GGTGA(8/7)
MvaI          222  CCAGG                CC^[AT]GG
NlaIII         10  CATG                 CATG^
PflMI         237  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI           46  GAAGAGC              GCTCTTC(1/4)
Van91I        237  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AspHI          49  GAGCTC               G[AT]GC[AT]^C
              412  GAGCTC              
BanII          49  GAGCTC               G[GA]GC[CT]^C
              412  GAGCTC              
BglI          389  GCCGCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              457  GCCTATGTGGC         
BmyI           49  GAGCTC               G[AGT]GC[ACT]^C
              412  GAGCTC              
BpmI          127  CTGGAG               CTGGAG(16/14)
              364  CTGGAG              
BsiHKAI        49  GAGCTC               G[AT]GC[AT]^C
              412  GAGCTC              
Bsp1286I       49  GAGCTC               G[AGT]GC[ACT]^C
              412  GAGCTC              
BstYI         368  AGATCC               [GA]^GATC[CT]
              489  GGATCT              
DdeI          198  CTCAG                C^T[ACGT]AG
              294  CTTAG               
DsaI          160  CCGCGG               C^C[GA][CT]GG
              390  CCGCGG              
EarI          418  CTCTTC               CTCTTC(1/4)
               46  GAAGAG              
HinfI         226  GAGTC                G^A[ACGT]TC
              438  GAGTC               
Ksp632I       418  CTCTTC               CTCTTC(1/4)
               46  GAAGAG              
KspI          160  CCGCGG               CCGC^GG
              390  CCGCGG              
MflI          368  AGATCC               [GA]^GATC[CT]
              489  GGATCT              
PleI          226  GAGTC                GAGTC(4/5)
              438  GAGTC               
SacI           49  GAGCTC               GAGCT^C
              412  GAGCTC              
SacII         160  CCGCGG               CCGC^GG
              390  CCGCGG              
TspRI         200  CAGTG                CA[GC]TG[ACGT][ACGT]^
              469  CACTG               
XhoII         368  AGATCC               [GA]^GATC[CT]
              489  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BbvI        
BcnI        BsaJI       BseRI       BsiYI       BslI        
BssKI       BstUI       Cac8I       CfoI        Cfr13I      
DpnII       FauI        Fnu4HI      HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MboI        MboII       MnlI        MspA1I      MspI        
MvnI        MwoI        NciI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       TseI