Restriction map for
pF1KE0274
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# NO CUT ENZYMES ( 187 enzymes) #
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Acc65I AccI AccII AccIII AclI
AfaI AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaJI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII Cfr10I Cfr13I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI Sau3AI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI TfiI Tsp45I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 96 GACGTC GACGT^C
AcyI 96 GACGTC G[GA]^CG[CT]C
AflII 190 CTTAAG C^TTAAG
AluI 188 AGCT AG^CT
Aor51HI 42 AGCGCT AGC^GCT
AspHI 16 GTGCTC G[AT]GC[AT]^C
AspI 234 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 220 GGTGCC G^G[CT][GA]CC
BfrI 190 CTTAAG C^TTAAG
BmyI 16 GTGCTC G[AGT]GC[ACT]^C
BsaHI 96 GACGTC G[GA]^CG[CT]C
BsaWI 143 TCCGGT [AT]^CCGG[AT]
BsiHKAI 16 GTGCTC G[AT]GC[AT]^C
BsiYI 309 CCAGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 309 CCAGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 16 GTGCTC G[AGT]GC[ACT]^C
BsrBI 165 GAGCGG CCGCTC(-3/-3)
BsrDI 33 CATTGC GCAATG(2/0)
BsrI 122 CCAGT ACTGG(1/-1)
CfoI 43 GCGC GCG^C
DdeI 19 CTCAG C^T[ACGT]AG
EarI 184 GAAGAG CTCTTC(1/4)
Eco47III 42 AGCGCT AGC^GCT
EcoRI 304 GAATTC G^AATTC
FauI 167 GCGGG CCCGC(4/6)
HaeII 42 AGCGCT [GA]GCGC^[CT]
HaeIII 103 GGCC GG^CC
HapII 144 CCGG C^CGG
HhaI 43 GCGC GCG^C
Hin1I 96 GACGTC G[GA]^CG[CT]C
HinP1I 43 GCGC G^CGC
HinfI 238 GAGTC G^A[ACGT]TC
HpaII 144 CCGG C^CGG
HphI 129 GGTGA GGTGA(8/7)
Ksp632I 184 GAAGAG CTCTTC(1/4)
MseI 191 TTAA T^TAA
MslI 9 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 144 CCGG C^CGG
NlaIV 220 GGTGCC GG[ACGT]^[ACGT]CC
PflFI 234 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 309 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 238 GAGTC GAGTC(4/5)
SapI 184 GAAGAGC GCTCTTC(1/4)
SfaNI 5 GCATC GCATC(5/9)
SmlI 190 CTTAAG C^T[CT][GA]AG
StuI 102 AGGCCT AGG^CCT
Tru9I 191 TTAA T^TAA
TspRI 21 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 234 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 309 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 248 CCAAGTCCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AcsI 85 AAATTC [GA]^AATT[CT]
304 GAATTC
ApoI 85 AAATTC [GA]^AATT[CT]
304 GAATTC
BpmI 46 CTCCAG CTGGAG(16/14)
202 CTCCAG
BsmI 52 GAATGC GAATGC(1/-1)
321 GCATTC
BssKI 48 CCAGG ^CC[ACGT]GG
309 CCAGG
BstF5I 135 GGATG GGATG(2/0)
177 GGATG
BstNI 48 CCAGG CC^[AT]GG
309 CCAGG
EcoRII 48 CCAGG ^CC[AT]GG
309 CCAGG
FokI 135 GGATG GGATG(9/13)
177 GGATG
MaeII 14 ACGT A^CGT
97 ACGT
MvaI 48 CCAGG CC^[AT]GG
309 CCAGG
ScrFI 48 CCAGG CC^[ACGT]GG
309 CCAGG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AciI BbvI Fnu4HI ItaI MboII
MnlI TseI Tsp509I