Restriction map for
pF1KE0269
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 28 enzymes) #
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ApaI 1176 GGGCCC GGGCC^C
Asp700I 578 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1168 CCTAGG C^CTAGG
BamHI 1102 GGATCC G^GATCC
BanII 1176 GGGCCC G[GA]GC[CT]^C
BbsI 1286 GAAGAC GAAGAC(2/6)
BlnI 1168 CCTAGG C^CTAGG
BpuAI 1286 GAAGAC GAAGAC(2/6)
BsaBI 274 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1127 GGTCTC GGTCTC(1/5)
BseRI 289 GAGGAG GAGGAG(10/8)
BsgI 826 GTGCAG GTGCAG(16/14)
BspHI 449 TCATGA T^CATGA
BspMI 1046 ACCTGC ACCTGC(4/8)
BstAPI 532 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 86 TTCGAA TT^CGAA
HinfI 262 GATTC G^A[ACGT]TC
MamI 274 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 536 CAGCTG C[AC]G^C[GT]G
NspV 86 TTCGAA TT^CGAA
PstI 931 CTGCAG CTGCA^G
PvuII 536 CAGCTG CAG^CTG
RcaI 449 TCATGA T^CATGA
SfcI 931 CTGCAG C^T[GA][CT]AG
SfuI 86 TTCGAA TT^CGAA
TfiI 262 GATTC G^A[AT]TC
XcmI 404 CCAAGACCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 578 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AciI 1051 CCGC CCGC(-3/-1)
370 GCGG
AcsI 21 AAATTT [GA]^AATT[CT]
780 GAATTT
ApoI 21 AAATTT [GA]^AATT[CT]
780 GAATTT
AspHI 989 GAGCAC G[AT]GC[AT]^C
1159 GAGCAC
AvaI 1082 CCCGGG C^[CT]CG[GA]G
1281 CCCGGG
AvaII 372 GGACC G^G[AT]CC
1020 GGACC
BanI 868 GGTGCC G^G[CT][GA]CC
1043 GGCACC
BpmI 949 CTGGAG CTGGAG(16/14)
1006 CTGGAG
BsiHKAI 989 GAGCAC G[AT]GC[AT]^C
1159 GAGCAC
BsmFI 517 GGGAC GGGAC(10/14)
123 GTCCC
BsoBI 1082 CCCGGG C^[CT]CG[GA]G
1281 CCCGGG
BsrFI 1022 ACCGGC [GA]^CCGG[CT]
1040 ACCGGC
BsrI 514 ACTGG ACTGG(1/-1)
612 ACTGG
BssSI 391 CACGAG CACGAG(-5/-1)
148 CTCGTG
BstXI 410 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
605 CCAGCCGACTGG
BstYI 723 GGATCT [GA]^GATC[CT]
1102 GGATCC
Cfr10I 1022 ACCGGC [GA]^CCGG[CT]
1040 ACCGGC
DpnII 724 GATC ^GATC
1103 GATC
EarI 426 CTCTTC CTCTTC(1/4)
238 GAAGAG
HincII 189 GTCAAC GT[CT]^[GA]AC
379 GTTGAC
HindII 189 GTCAAC GT[CT]^[GA]AC
379 GTTGAC
Ksp632I 426 CTCTTC CTCTTC(1/4)
238 GAAGAG
MboI 724 GATC ^GATC
1103 GATC
MflI 723 GGATCT [GA]^GATC[CT]
1102 GGATCC
MslI 199 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
765 CATGGCTATG
NdeII 724 GATC ^GATC
1103 GATC
SapI 238 GAAGAGC GCTCTTC(1/4)
425 GCTCTTC
Sau3AI 724 GATC ^GATC
1103 GATC
SmaI 1082 CCCGGG CCC^GGG
1281 CCCGGG
Tsp45I 254 GTGAC ^GT[GC]AC
481 GTGAC
TspRI 201 CAGTG CA[GC]TG[ACGT][ACGT]^
1190 CACTG
XhoII 723 GGATCT [GA]^GATC[CT]
1102 GGATCC
XmaCI 1082 CCCGGG C^CCGGG
1281 CCCGGG
XmaI 1082 CCCGGG C^CCGGG
1281 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AluI AlwI AlwNI BalI BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeI MaeIII MboII MluNI
MnlI MscI MseI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp509I TthHB8I