##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MboII       
MfeI        MluI        MluNI       MscI        MseI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tru9I       
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccIII        123  TCCGGA               T^CCGGA
AfaI           13  GTAC                 GT^AC
AluI          135  AGCT                 AG^CT
AlwI          168  GGATC                GGATC(4/5)
AvaII         256  GGACC                G^G[AT]CC
BfaI           46  CTAG                 C^TAG
BglII         263  AGATCT               A^GATCT
BsaWI         123  TCCGGA               [AT]^CCGG[AT]
BseAI         123  TCCGGA               T^CCGGA
BspEI         123  TCCGGA               T^CCGGA
BssKI         165  CCAGG                ^CC[ACGT]GG
BstNI         165  CCAGG                CC^[AT]GG
BstYI         263  AGATCT               [GA]^GATC[CT]
Cac8I          63  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr13I        256  GGACC                G^G[ACGT]CC
EcoRII        165  CCAGG                ^CC[AT]GG
HapII         124  CCGG                 C^CGG
HinfI         276  GATTC                G^A[ACGT]TC
HpaII         124  CCGG                 C^CGG
HphI           23  TCACC                GGTGA(8/7)
MaeI           46  CTAG                 C^TAG
MaeII         245  ACGT                 A^CGT
MflI          263  AGATCT               [GA]^GATC[CT]
MnlI           75  CCTC                 CCTC(7/6)
MroI          123  TCCGGA               T^CCGGA
MspI          124  CCGG                 C^CGG
MvaI          165  CCAGG                CC^[AT]GG
NlaIII         60  CATG                 CATG^
RsaI           13  GTAC                 GT^AC
Sau96I        256  GGACC                G^G[ACGT]CC
ScrFI         165  CCAGG                CC^[ACGT]GG
SfcI           84  CTACAG               C^T[GA][CT]AG
TfiI          276  GATTC                G^A[AT]TC
Tsp45I        113  GTCAC                ^GT[GC]AC
Tsp509I       224  AATT                 ^AATT
XhoII         263  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BbvI           42  GCTGC                GCAGC(8/12)
              233  GCTGC               
BstF5I        180  GGATG                GGATG(2/0)
              190  GGATG               
Fnu4HI         42  GCTGC                GC^[ACGT]GC
              233  GCTGC               
FokI          180  GGATG                GGATG(9/13)
              190  GGATG               
ItaI           42  GCTGC                GC^[ACGT]GC
              233  GCTGC               
MaeIII        113  GTCAC                ^GT[ACGT]AC
              206  GTTAC               
TseI           42  GCTGC                G^C[AT]GC
              233  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

DpnII       MboI        MwoI        NdeII       Sau3AI