##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        DsaI        Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FokI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AccII         245  CGCG                 CG^CG
AvaII         201  GGTCC                G^G[AT]CC
BsaI          348  GGTCTC               GGTCTC(1/5)
BseRI          50  CTCCTC               GAGGAG(10/8)
BsiEI         114  CGGCCG               CG[GA][CT]^CG
BsmAI         349  GTCTC                GTCTC(1/5)
BsrBI          65  CCGCTC               CCGCTC(-3/-3)
BstUI         245  CGCG                 CG^CG
BstXI         392  CCAATGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI          114  CGGCCG               C^GGCCG
EclXI         114  CGGCCG               C^GGCCG
Eco52I        114  CGGCCG               C^GGCCG
FauI           88  CCCGC                CCCGC(4/6)
HaeII         139  AGCGCC               [GA]GCGC^[CT]
HgaI            7  GCGTC                GACGC(5/10)
HphI          170  TCACC                GGTGA(8/7)
MboII         374  TCTTC                GAAGA(8/7)
MspA1I         17  CAGCTG               C[AC]G^C[GT]G
MvnI          245  CGCG                 CG^CG
NlaIII        367  CATG                 CATG^
PpuMI         200  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII          17  CAGCTG               CAG^CTG
SacI          269  GAGCTC               GAGCT^C
StuI           97  AGGCCT               AGG^CCT
Tsp509I       404  AATT                 ^AATT
TspRI          28  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI           18  AGCT                 AG^CT
              270  AGCT                
AspHI         239  GTGCTC               G[AT]GC[AT]^C
              269  GAGCTC              
BsiHKAI       239  GTGCTC               G[AT]GC[AT]^C
              269  GAGCTC              
CfoI          140  GCGC                 GCG^C
              151  GCGC                
EaeI           11  CGGCCA               [CT]^GGCC[GA]
              114  CGGCCG              
HhaI          140  GCGC                 GCG^C
              151  GCGC                
HinP1I        140  GCGC                 G^CGC
              151  GCGC                
SmaI          158  CCCGGG               CCC^GGG
              317  CCCGGG              
XmaCI         158  CCCGGG               C^CCGGG
              317  CCCGGG              
XmaI          158  CCCGGG               C^CCGGG
              317  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        ApaI        AvaI        BanI        BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsoBI       Bsp1286I    BssKI       BstNI       
Cac8I       Cfr13I      DraII       EcoO109I    EcoRII      
Fnu4HI      HaeIII      HapII       HpaII       ItaI        
MnlI        MspI        MvaI        MwoI        NciI        
NlaIV       Sau96I      ScrFI       TseI