Restriction map for
pF1KE0256
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 335 ACGTGT A^C[GA][CT]GT
AlwNI 270 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1144 CCTCAGG CC^T[ACGT]AGG
AvaII 368 GGTCC G^G[AT]CC
BanI 520 GGCACC G^G[CT][GA]CC
BanII 881 GGGCTC G[GA]GC[CT]^C
BclI 933 TGATCA T^GATCA
BmyI 881 GGGCTC G[AGT]GC[ACT]^C
BpmI 596 CTCCAG CTGGAG(16/14)
BsaAI 502 TACGTG [CT]AC^GT[GA]
BsaWI 402 ACCGGA [AT]^CCGG[AT]
BsgI 874 CTGCAC GTGCAG(16/14)
Bsp1286I 881 GGGCTC G[AGT]GC[ACT]^C
BspMI 287 ACCTGC ACCTGC(4/8)
BsrDI 720 CATTGC GCAATG(2/0)
BstYI 43 GGATCT [GA]^GATC[CT]
Bsu36I 1144 CCTCAGG CC^T[ACGT]AGG
Eco81I 1144 CCTCAGG CC^T[ACGT]AGG
EcoNI 1128 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 850 GCGGG CCCGC(4/6)
FbaI 933 TGATCA T^GATCA
HapII 403 CCGG C^CGG
HindIII 911 AAGCTT A^AGCTT
HpaII 403 CCGG C^CGG
MflI 43 GGATCT [GA]^GATC[CT]
MslI 726 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 403 CCGG C^CGG
MwoI 532 GCTTTTATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 22 GCATGC [GA]CATG^[CT]
PflMI 269 CCAGTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SphI 22 GCATGC GCATG^C
TaqI 741 TCGA T^CGA
TthHB8I 741 TCGA T^CGA
Van91I 269 CCAGTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 43 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 799 CCGC CCGC(-3/-1)
850 GCGG
BssKI 211 CCAGG ^CC[ACGT]GG
653 CCAGG
BstNI 211 CCAGG CC^[AT]GG
653 CCAGG
Cac8I 22 GCATGC GC[ACGT]^[ACGT]GC
231 GCCTGC
EarI 139 GAAGAG CTCTTC(1/4)
200 GAAGAG
EcoRII 211 CCAGG ^CC[AT]GG
653 CCAGG
HaeIII 414 GGCC GG^CC
508 GGCC
Ksp632I 139 GAAGAG CTCTTC(1/4)
200 GAAGAG
MaeII 335 ACGT A^CGT
503 ACGT
MvaI 211 CCAGG CC^[AT]GG
653 CCAGG
NlaIV 520 GGCACC GG[ACGT]^[ACGT]CC
594 GGCTCC
PleI 494 GACTC GAGTC(4/5)
816 GAGTC
ScrFI 211 CCAGG CC^[ACGT]GG
653 CCAGG
SspI 100 AATATT AAT^ATT
1184 AATATT
TfiI 189 GATTC G^A[AT]TC
1098 GATTC
TspRI 590 CACTG CA[GC]TG[ACGT][ACGT]^
877 CACTG
XcmI 293 CCAACAACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1000 CCAACAGCAATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BsaJI BsiYI BslI BsrI BstF5I
Cfr13I DdeI DpnII EcoT14I Fnu4HI
FokI HinfI HphI ItaI MaeIII
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI Sau96I SfaNI StyI
Tru9I TseI Tsp45I Tsp509I