Restriction map for
pF1KE0252
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# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 525 CCGC CCGC(-3/-1)
AfaI 304 GTAC GT^AC
AflIII 324 ACGTGT A^C[GA][CT]GT
AlwNI 13 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
BbvI 120 GCTGC GCAGC(8/12)
BpmI 574 CTCCAG CTGGAG(16/14)
BsgI 10 GTGCAG GTGCAG(16/14)
BsiYI 264 CCACACCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 264 CCACACCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 439 GCAGGT ACCTGC(4/8)
BsrDI 379 GCAATG GCAATG(2/0)
BstXI 512 CCATTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 12 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 16 GCGC GCG^C
DraII 71 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 261 CGGCCA [CT]^GGCC[GA]
EcoO109I 71 GGGACCC [GA]G^G[ACGT]CC[CT]
Fnu4HI 120 GCTGC GC^[ACGT]GC
HaeII 15 GGCGCT [GA]GCGC^[CT]
HhaI 16 GCGC GCG^C
HinP1I 16 GCGC G^CGC
HinfI 338 GAATC G^A[ACGT]TC
ItaI 120 GCTGC GC^[ACGT]GC
MaeII 324 ACGT A^CGT
MboII 277 GAAGA GAAGA(8/7)
NspI 470 ACATGC [GA]CATG^[CT]
PflMI 264 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 71 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 304 GTAC GT^AC
SfcI 171 CTACAG C^T[GA][CT]AG
SspI 554 AATATT AAT^ATT
TfiI 338 GAATC G^A[AT]TC
TseI 120 GCTGC G^C[AT]GC
Tsp509I 518 AATT ^AATT
Van91I 264 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 50 CCATTTTGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 72 GGACC G^G[AT]CC
522 GGACC
BanI 21 GGTGCC G^G[CT][GA]CC
95 GGTGCC
BmyI 22 GTGCCC G[AGT]GC[ACT]^C
96 GTGCCC
BsaJI 136 CCAAGG C^C[ACGT][ACGT]GG
269 CCTTGG
BsmFI 71 GGGAC GGGAC(10/14)
642 GTCCC
Bsp1286I 22 GTGCCC G[AGT]GC[ACT]^C
96 GTGCCC
BstYI 353 GGATCT [GA]^GATC[CT]
411 GGATCT
DdeI 186 CTTAG C^T[ACGT]AG
357 CTCAG
EcoT14I 136 CCAAGG C^C[AT][AT]GG
269 CCTTGG
HphI 246 GGTGA GGTGA(8/7)
253 GGTGA
MaeIII 401 GTGAC ^GT[ACGT]AC
503 GTGAC
MflI 353 GGATCT [GA]^GATC[CT]
411 GGATCT
MnlI 364 CCTC CCTC(7/6)
68 GAGG
MslI 89 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
619 CATGTGGATG
NlaIII 471 CATG CATG^
619 CATG
StyI 136 CCAAGG C^C[AT][AT]GG
269 CCTTGG
Tsp45I 401 GTGAC ^GT[GC]AC
503 GTGAC
XhoII 353 GGATCT [GA]^GATC[CT]
411 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AlwI BfaI BsrI BssKI
BstF5I BstNI Cfr13I DpnII EcoRII
FokI HaeIII MaeI MboI MseI
MvaI NdeII NlaIV Sau3AI Sau96I
ScrFI Tru9I TspRI