Restriction map for
pF1KE0239
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 514 GAATTC [GA]^AATT[CT]
AocI 71 CCTCAGG CC^T[ACGT]AGG
ApoI 514 GAATTC [GA]^AATT[CT]
AvaII 153 GGACC G^G[AT]CC
BanI 658 GGTGCC G^G[CT][GA]CC
BanII 19 GAGCTC G[GA]GC[CT]^C
BbsI 362 GTCTTC GAAGAC(2/6)
BbvCI 381 CCTCAGC CCTCAGC(-5/-2)
BbvI 132 GCTGC GCAGC(8/12)
BlpI 243 GCTCAGC GC^T[ACGT]AGC
BpmI 146 CTGGAG CTGGAG(16/14)
Bpu1102I 243 GCTCAGC GC^T[ACGT]AGC
BpuAI 362 GTCTTC GAAGAC(2/6)
BsaI 107 GAGACC GGTCTC(1/5)
BsaJI 339 CCACGG C^C[ACGT][ACGT]GG
BsgI 270 CTGCAC GTGCAG(16/14)
BsmI 248 GCATTC GAATGC(1/-1)
BspMI 268 ACCTGC ACCTGC(4/8)
BssKI 391 CCAGG ^CC[ACGT]GG
BstNI 391 CCAGG CC^[AT]GG
Bsu36I 71 CCTCAGG CC^T[ACGT]AGG
Cac8I 565 GCCAGC GC[ACGT]^[ACGT]GC
CelII 243 GCTCAGC GC^T[ACGT]AGC
Cfr13I 153 GGACC G^G[ACGT]CC
DpnII 523 GATC ^GATC
DsaI 339 CCACGG C^C[GA][CT]GG
Eco81I 71 CCTCAGG CC^T[ACGT]AGG
EcoNI 67 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 514 GAATTC G^AATTC
EcoRII 391 CCAGG ^CC[AT]GG
EcoRV 289 GATATC GAT^ATC
Fnu4HI 132 GCTGC GC^[ACGT]GC
HaeIII 635 GGCC GG^CC
ItaI 132 GCTGC GC^[ACGT]GC
MboI 523 GATC ^GATC
MseI 685 TTAA T^TAA
MvaI 391 CCAGG CC^[AT]GG
NdeII 523 GATC ^GATC
PleI 645 GACTC GAGTC(4/5)
PstI 26 CTGCAG CTGCA^G
SacI 19 GAGCTC GAGCT^C
SapI 809 GAAGAGC GCTCTTC(1/4)
Sau3AI 523 GATC ^GATC
Sau96I 153 GGACC G^G[ACGT]CC
ScrFI 391 CCAGG CC^[ACGT]GG
TfiI 262 GAATC G^A[AT]TC
Tru9I 685 TTAA T^TAA
TseI 132 GCTGC G^C[AT]GC
Tsp509I 515 AATT ^AATT
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 354 GTAC GT^AC
754 GTAC
AluI 20 AGCT AG^CT
402 AGCT
Alw44I 121 GTGCAC G^TGCAC
331 GTGCAC
ApaLI 121 GTGCAC G^TGCAC
331 GTGCAC
BfaI 115 CTAG C^TAG
652 CTAG
BseRI 795 GAGGAG GAGGAG(10/8)
798 GAGGAG
BsiYI 67 CCTGCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
339 CCACGGTCAGG
BslI 67 CCTGCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
339 CCACGGTCAGG
BsmAI 88 GAGAC GTCTC(1/5)
107 GAGAC
BsrI 302 ACTGG ACTGG(1/-1)
140 CCAGT
HinfI 262 GAATC G^A[ACGT]TC
645 GACTC
HphI 678 GGTGA GGTGA(8/7)
320 TCACC
MaeI 115 CTAG C^TAG
652 CTAG
NlaIV 535 GGAACC GG[ACGT]^[ACGT]CC
658 GGTGCC
RsaI 354 GTAC GT^AC
754 GTAC
SfaNI 165 GATGC GCATC(5/9)
771 GCATC
TaqI 265 TCGA T^CGA
521 TCGA
TthHB8I 265 TCGA T^CGA
521 TCGA
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
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AspHI BmyI BsiHKAI Bsp1286I BstF5I
DdeI EarI FokI Ksp632I MboII
MnlI MslI MwoI NlaIII SfcI
TspRI