Restriction map for
pF1KE0235
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FauI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI ItaI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TseI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AciI 793 CCGC CCGC(-3/-1)
AcsI 192 GAATTT [GA]^AATT[CT]
AflIII 921 ACATGT A^C[GA][CT]GT
ApaI 780 GGGCCC GGGCC^C
ApoI 192 GAATTT [GA]^AATT[CT]
AvaII 757 GGACC G^G[AT]CC
BclI 804 TGATCA T^GATCA
BsmAI 742 GTCTC GTCTC(1/5)
BspLU11I 921 ACATGT A^CATGT
BsrDI 20 CATTGC GCAATG(2/0)
BssKI 84 CCTGG ^CC[ACGT]GG
BstNI 84 CCTGG CC^[AT]GG
Cac8I 795 GCAAGC GC[ACGT]^[ACGT]GC
DraII 780 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 326 CCACGG C^C[GA][CT]GG
EcoO109I 780 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 84 CCTGG ^CC[AT]GG
EcoT14I 186 CCTTGG C^C[AT][AT]GG
FbaI 804 TGATCA T^GATCA
HaeIII 781 GGCC GG^CC
HapII 426 CCGG C^CGG
HpaII 426 CCGG C^CGG
MspI 426 CCGG C^CGG
MvaI 84 CCTGG CC^[AT]GG
MwoI 346 GCAACGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 921 ACATGT [GA]CATG^[CT]
ScrFI 84 CCTGG CC^[ACGT]GG
SfcI 867 CTACAG C^T[GA][CT]AG
StyI 186 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 198 AGCT AG^CT
258 AGCT
AseI 432 ATTAAT AT^TAAT
580 ATTAAT
AsnI 432 ATTAAT AT^TAAT
580 ATTAAT
Asp700I 246 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
331 GAATGTTTTC
BanII 725 GAGCCC G[GA]GC[CT]^C
780 GGGCCC
BmyI 725 GAGCCC G[AGT]GC[ACT]^C
780 GGGCCC
BsaJI 186 CCTTGG C^C[ACGT][ACGT]GG
326 CCACGG
BsiYI 78 CCTGTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
113 CCATCATGGGG
BslI 78 CCTGTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
113 CCATCATGGGG
BsmI 286 GAATGC GAATGC(1/-1)
88 GCATTC
Bsp1286I 725 GAGCCC G[AGT]GC[ACT]^C
780 GGGCCC
DpnII 361 GATC ^GATC
805 GATC
EcoRV 216 GATATC GAT^ATC
273 GATATC
HinfI 123 GAATC G^A[ACGT]TC
571 GATTC
HphI 110 TCACC GGTGA(8/7)
159 TCACC
MboI 361 GATC ^GATC
805 GATC
NdeII 361 GATC ^GATC
805 GATC
PshBI 432 ATTAAT AT^TAAT
580 ATTAAT
Sau3AI 361 GATC ^GATC
805 GATC
TfiI 123 GAATC G^A[AT]TC
571 GATTC
XmnI 246 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
331 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI BstF5I Cfr13I DdeI EarI
FokI Ksp632I MaeIII MboII MnlI
MseI MslI NdeI NlaIII NlaIV
RsaI Sau96I SfaNI Tru9I Tsp45I
Tsp509I TspRI