Restriction map for
pF1KE0232
##################################
# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I BspDI
BspEI BsrBI BsrFI BssHII BssKI
BssSI Bst1107I BstBI BstNI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRII EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MroI MspA1I MspI MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AciI 357 CCGC CCGC(-3/-1)
AflIII 98 ACATGT A^C[GA][CT]GT
BfaI 625 CTAG C^TAG
BglII 551 AGATCT A^GATCT
BpmI 484 CTCCAG CTGGAG(16/14)
BsmAI 205 GTCTC GTCTC(1/5)
BsmI 224 GCATTC GAATGC(1/-1)
BspHI 398 TCATGA T^CATGA
BspLU11I 98 ACATGT A^CATGT
BsrDI 741 CATTGC GCAATG(2/0)
BsrI 287 CCAGT ACTGG(1/-1)
BstAPI 63 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 49 GGTGACC G^GT[ACGT]ACC
BstF5I 719 CATCC GGATG(2/0)
BstPI 49 GGTGACC G^GT[ACGT]ACC
BstYI 551 AGATCT [GA]^GATC[CT]
Cac8I 445 GCTGGC GC[ACGT]^[ACGT]GC
DraII 537 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 537 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 49 GGTGACC G^GT[ACGT]ACC
EcoRI 493 GAATTC G^AATTC
FokI 719 CATCC GGATG(9/13)
HindIII 890 AAGCTT A^AGCTT
MaeI 625 CTAG C^TAG
MboII 761 TCTTC GAAGA(8/7)
MflI 551 AGATCT [GA]^GATC[CT]
MseI 158 TTAA T^TAA
NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC
NspI 98 ACATGT [GA]CATG^[CT]
PpuMI 537 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 398 TCATGA T^CATGA
SfcI 215 CTACAG C^T[GA][CT]AG
SmlI 867 CTTGAG C^T[CT][GA]AG
SspI 294 AATATT AAT^ATT
TaqI 491 TCGA T^CGA
Tru9I 158 TTAA T^TAA
TspRI 897 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 491 TCGA T^CGA
XhoII 551 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 147 GGACC G^G[AT]CC
538 GGTCC
BalI 365 TGGCCA TGG^CCA
797 TGGCCA
BbvI 340 GCAGC GCAGC(8/12)
755 GCTGC
BclI 455 TGATCA T^GATCA
516 TGATCA
BsiYI 135 CCTCACATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
357 CCGCTATGTGG
BslI 135 CCTCACATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
357 CCGCTATGTGG
BsmFI 39 GGGAC GGGAC(10/14)
257 GGGAC
Bsp1407I 239 TGTACA T^GTACA
650 TGTACA
BspMI 59 ACCTGC ACCTGC(4/8)
535 GCAGGT
BsrGI 239 TGTACA T^GTACA
650 TGTACA
Cfr13I 147 GGACC G^G[ACGT]CC
538 GGTCC
EaeI 365 TGGCCA [CT]^GGCC[GA]
797 TGGCCA
FbaI 455 TGATCA T^GATCA
516 TGATCA
Fnu4HI 340 GCAGC GC^[ACGT]GC
755 GCTGC
ItaI 340 GCAGC GC^[ACGT]GC
755 GCTGC
MluNI 365 TGGCCA TGG^CCA
797 TGGCCA
MscI 365 TGGCCA TGG^CCA
797 TGGCCA
MwoI 63 GCAAGTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
601 GCTGTATTTGC
PleI 502 GACTC GAGTC(4/5)
901 GACTC
Sau96I 147 GGACC G^G[ACGT]CC
538 GGTCC
SfaNI 532 GATGC GCATC(5/9)
758 GCATC
SspBI 239 TGTACA T^GTACA
650 TGTACA
StuI 480 AGGCCT AGG^CCT
701 AGGCCT
TfiI 233 GATTC G^A[AT]TC
636 GATTC
TseI 340 GCAGC G^C[AT]GC
755 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
DdeI DpnII HaeIII HinfI HphI
MaeIII MboI MnlI MslI NdeII
NlaIII RsaI Sau3AI Tsp45I Tsp509I
XmnI