Restriction map for
pF1KE0231
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MaeIII MluI MluNI MroI MscI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 628 GTAGAC GT^[AC][GT]AC
AcsI 307 GAATTC [GA]^AATT[CT]
AflII 706 CTTAAG C^TTAAG
ApaI 175 GGGCCC GGGCC^C
ApoI 307 GAATTC [GA]^AATT[CT]
AseI 817 ATTAAT AT^TAAT
AsnI 817 ATTAAT AT^TAAT
AspHI 611 GAGCAC G[AT]GC[AT]^C
BanI 133 GGCACC G^G[CT][GA]CC
BbvI 101 GCAGC GCAGC(8/12)
BfrI 706 CTTAAG C^TTAAG
BpmI 334 CTGGAG CTGGAG(16/14)
BsaBI 84 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 700 GAGGAG GAGGAG(10/8)
BsgI 740 GTGCAG GTGCAG(16/14)
BsiHKAI 611 GAGCAC G[AT]GC[AT]^C
BspHI 557 TCATGA T^CATGA
BspMI 758 ACCTGC ACCTGC(4/8)
BsrDI 642 CATTGC GCAATG(2/0)
BsrI 333 ACTGG ACTGG(1/-1)
BstYI 83 GGATCT [GA]^GATC[CT]
EaeI 776 TGGCCG [CT]^GGCC[GA]
EcoRI 307 GAATTC G^AATTC
Fnu4HI 101 GCAGC GC^[ACGT]GC
HindIII 498 AAGCTT A^AGCTT
HphI 32 TCACC GGTGA(8/7)
ItaI 101 GCAGC GC^[ACGT]GC
MamI 84 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 869 CAATTG C^AATTG
MflI 83 GGATCT [GA]^GATC[CT]
MspA1I 856 CCGCTG C[AC]G^C[GT]G
MunI 869 CAATTG C^AATTG
NdeI 20 CATATG CA^TATG
PleI 337 GAGTC GAGTC(4/5)
PshAI 414 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 817 ATTAAT AT^TAAT
PstI 99 CTGCAG CTGCA^G
RcaI 557 TCATGA T^CATGA
ScaI 330 AGTACT AGT^ACT
SfcI 99 CTGCAG C^T[GA][CT]AG
SphI 461 GCATGC GCATG^C
TseI 101 GCAGC G^C[AT]GC
XhoII 83 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 789 CCGC CCGC(-3/-1)
856 CCGC
AluI 499 AGCT AG^CT
704 AGCT
AlwI 663 GATCC GGATC(4/5)
83 GGATC
BanII 160 GAGCCC G[GA]GC[CT]^C
175 GGGCCC
BclI 353 TGATCA T^GATCA
746 TGATCA
BcnI 679 CCGGG CC^[GC]GG
797 CCGGG
BsaJI 58 CCAGGG C^C[ACGT][ACGT]GG
302 CCCAGG
BsiYI 167 CCTTGCATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
431 CCCAAATAGGG
BslI 167 CCTTGCATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
431 CCCAAATAGGG
BsmAI 542 GTCTC GTCTC(1/5)
805 GTCTC
Cac8I 292 GCCTGC GC[ACGT]^[ACGT]GC
461 GCATGC
Cfr13I 175 GGGCC G^G[ACGT]CC
176 GGCCC
FbaI 353 TGATCA T^GATCA
746 TGATCA
HapII 679 CCGG C^CGG
797 CCGG
HpaII 679 CCGG C^CGG
797 CCGG
MspI 679 CCGG C^CGG
797 CCGG
MwoI 162 GCCCTCCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
292 GCCTGCTTTGC
NciI 679 CCGGG CC^[GC]GG
797 CCGGG
NlaIV 133 GGCACC GG[ACGT]^[ACGT]CC
175 GGGCCC
Sau96I 175 GGGCC G^G[ACGT]CC
176 GGCCC
TfiI 323 GATTC G^A[AT]TC
371 GATTC
TspRI 243 CACTG CA[GC]TG[ACGT][ACGT]^
743 CAGTG
XcmI 35 CCACCTTCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
764 CCATCATCAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI BfaI BmyI Bsp1286I BssKI
BstF5I BstNI DdeI DpnII EcoRII
FokI HaeIII HinfI MaeI MboI
MboII MnlI MseI MslI MvaI
NdeII NlaIII NspI RsaI Sau3AI
ScrFI SfaNI SmlI Tru9I Tsp509I