Restriction map for
pF1KE0187
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRII
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MaeIII MfeI MluI
MroI MslI MspA1I MunI MvaI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 582 GTCTAC GT^[AC][GT]AC
AccII 72 CGCG CG^CG
AcsI 652 GAATTC [GA]^AATT[CT]
AfaI 550 GTAC GT^AC
AluI 150 AGCT AG^CT
ApaI 12 GGGCCC GGGCC^C
ApoI 652 GAATTC [GA]^AATT[CT]
AvaII 349 GGTCC G^G[AT]CC
BanII 12 GGGCCC G[GA]GC[CT]^C
BbvCI 62 CCTCAGC CCTCAGC(-5/-2)
BglII 78 AGATCT A^GATCT
BmyI 12 GGGCCC G[AGT]GC[ACT]^C
BsaBI 202 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 408 GGTCTC GGTCTC(1/5)
BseRI 60 CTCCTC GAGGAG(10/8)
BsmFI 44 GGGAC GGGAC(10/14)
Bsp1286I 12 GGGCCC G[AGT]GC[ACT]^C
BstF5I 98 CATCC GGATG(2/0)
BstUI 72 CGCG CG^CG
BstXI 495 CCAAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 78 AGATCT [GA]^GATC[CT]
DraII 348 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 671 CTCTTC CTCTTC(1/4)
EcoO109I 348 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 652 GAATTC G^AATTC
EcoRV 381 GATATC GAT^ATC
EcoT14I 501 CCTTGG C^C[AT][AT]GG
FauI 15 CCCGC CCCGC(4/6)
FokI 98 CATCC GGATG(9/13)
HgaI 70 GACGC GACGC(5/10)
HinfI 142 GAATC G^A[ACGT]TC
Ksp632I 671 CTCTTC CTCTTC(1/4)
MamI 202 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 78 AGATCT [GA]^GATC[CT]
MvnI 72 CGCG CG^CG
MwoI 619 GCCTACAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 488 CATATG CA^TATG
PflMI 298 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 348 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 550 GTAC GT^AC
StyI 501 CCTTGG C^C[AT][AT]GG
TfiI 142 GAATC G^A[AT]TC
Van91I 298 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 78 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 16 CCGC CCGC(-3/-1)
73 GCGG
AlwI 208 GATCC GGATC(4/5)
565 GGATC
BalI 461 TGGCCA TGG^CCA
492 TGGCCA
BcnI 10 CCGGG CC^[GC]GG
21 CCCGG
BsaJI 20 CCCCGG C^C[ACGT][ACGT]GG
501 CCTTGG
BsrI 459 ACTGG ACTGG(1/-1)
724 ACTGG
BssKI 10 CCGGG ^CC[ACGT]GG
21 CCCGG
Cac8I 14 GCCCGC GC[ACGT]^[ACGT]GC
182 GCTGGC
EaeI 461 TGGCCA [CT]^GGCC[GA]
492 TGGCCA
HapII 10 CCGG C^CGG
22 CCGG
HpaII 10 CCGG C^CGG
22 CCGG
MluNI 461 TGGCCA TGG^CCA
492 TGGCCA
MscI 461 TGGCCA TGG^CCA
492 TGGCCA
MseI 115 TTAA T^TAA
311 TTAA
MspI 10 CCGG C^CGG
22 CCGG
NciI 10 CCGGG CC^[GC]GG
21 CCCGG
NlaIII 378 CATG CATG^
413 CATG
NlaIV 12 GGGCCC GG[ACGT]^[ACGT]CC
348 GGGTCC
ScrFI 10 CCGGG CC^[ACGT]GG
21 CCCGG
SfaNI 288 GATGC GCATC(5/9)
97 GCATC
SfcI 146 CTACAG C^T[GA][CT]AG
621 CTACAG
Tru9I 115 TTAA T^TAA
311 TTAA
TspRI 84 CAGTG CA[GC]TG[ACGT][ACGT]^
624 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
BbvI BsiYI BslI BsmAI Cfr13I
DdeI DpnII Fnu4HI HaeIII HphI
ItaI MboI MboII MnlI NdeII
Sau3AI Sau96I TseI Tsp509I