Restriction map for
pF1KE0183
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmFI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I KasI KpnI KspI MamI
MfeI MroI MspA1I MunI NarI
NciI NdeI NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 943 CGCG CG^CG
AciI 639 CCGC CCGC(-3/-1)
AflIII 942 ACGCGT A^C[GA][CT]GT
Alw44I 791 GTGCAC G^TGCAC
AlwNI 710 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 791 GTGCAC G^TGCAC
Asp700I 771 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 791 GTGCAC G[AT]GC[AT]^C
AvaI 130 CTCGAG C^[CT]CG[GA]G
BalI 722 TGGCCA TGG^CCA
BanI 469 GGTGCC G^G[CT][GA]CC
BfaI 487 CTAG C^TAG
BpmI 5 CTGGAG CTGGAG(16/14)
BsiHKAI 791 GTGCAC G[AT]GC[AT]^C
BsmBI 413 GAGACG CGTCTC(1/5)
BsmI 611 GCATTC GAATGC(1/-1)
BsoBI 130 CTCGAG C^[CT]CG[GA]G
BsrFI 342 GCCGGC [GA]^CCGG[CT]
BstUI 943 CGCG CG^CG
BstYI 914 AGATCC [GA]^GATC[CT]
Cfr10I 342 GCCGGC [GA]^CCGG[CT]
DsaI 781 CCATGG C^C[GA][CT]GG
EaeI 722 TGGCCA [CT]^GGCC[GA]
EarI 771 GAAGAG CTCTTC(1/4)
EcoT14I 781 CCATGG C^C[AT][AT]GG
EcoT22I 497 ATGCAT ATGCA^T
HapII 343 CCGG C^CGG
HincII 114 GTTAAC GT[CT]^[GA]AC
HindII 114 GTTAAC GT[CT]^[GA]AC
HindIII 888 AAGCTT A^AGCTT
HpaI 114 GTTAAC GTT^AAC
HpaII 343 CCGG C^CGG
Ksp632I 771 GAAGAG CTCTTC(1/4)
MaeI 487 CTAG C^TAG
MflI 914 AGATCC [GA]^GATC[CT]
MluI 942 ACGCGT A^CGCGT
MluNI 722 TGGCCA TGG^CCA
MscI 722 TGGCCA TGG^CCA
MspI 343 CCGG C^CGG
MvnI 943 CGCG CG^CG
NaeI 342 GCCGGC GCC^GGC
NcoI 781 CCATGG C^CATGG
NgoMIV 342 GCCGGC G^CCGGC
NsiI 497 ATGCAT ATGCA^T
NspI 510 GCATGT [GA]CATG^[CT]
PaeR7I 130 CTCGAG C^TCGAG
SmlI 130 CTCGAG C^T[CT][GA]AG
SspI 574 AATATT AAT^ATT
StyI 781 CCATGG C^C[AT][AT]GG
XcmI 695 CCAGGATTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 130 CTCGAG C^TCGAG
XhoII 914 AGATCC [GA]^GATC[CT]
XmnI 771 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 915 GATCC GGATC(4/5)
48 GGATC
AseI 571 ATTAAT AT^TAAT
582 ATTAAT
AsnI 571 ATTAAT AT^TAAT
582 ATTAAT
BmyI 791 GTGCAC G[AGT]GC[ACT]^C
851 GTGCCC
BsmAI 413 GAGAC GTCTC(1/5)
514 GTCTC
Bsp1286I 791 GTGCAC G[AGT]GC[ACT]^C
851 GTGCCC
Cac8I 342 GCCGGC GC[ACGT]^[ACGT]GC
906 GCAAGC
DdeI 863 CTCAG C^T[ACGT]AG
911 CTAAG
DpnII 49 GATC ^GATC
915 GATC
HaeIII 723 GGCC GG^CC
897 GGCC
HgaI 415 GACGC GACGC(5/10)
941 GACGC
HphI 250 GGTGA GGTGA(8/7)
517 TCACC
MboI 49 GATC ^GATC
915 GATC
NdeII 49 GATC ^GATC
915 GATC
PleI 491 GACTC GAGTC(4/5)
649 GAGTC
PshBI 571 ATTAAT AT^TAAT
582 ATTAAT
Sau3AI 49 GATC ^GATC
915 GATC
TaqI 42 TCGA T^CGA
131 TCGA
TfiI 226 GATTC G^A[AT]TC
699 GATTC
Tsp45I 619 GTCAC ^GT[GC]AC
883 GTGAC
TthHB8I 42 TCGA T^CGA
131 TCGA
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
EcoRII Fnu4HI FokI HinfI ItaI
MaeII MaeIII MboII MnlI MseI
MslI MvaI MwoI NlaIII NlaIV
RsaI ScrFI Tru9I TseI Tsp509I
TspRI