Restriction map for
pF1KE0179
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI AlwI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BamHI
BanI BbeI BbrPI BbvCI BciVI
BfrI BglII BsaAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI MaeII MamI
MfeI MflI MluI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 595 GTCTAC GT^[AC][GT]AC
AccII 420 CGCG CG^CG
AccIII 137 TCCGGA T^CCGGA
AcsI 956 GAATTC [GA]^AATT[CT]
AfaI 231 GTAC GT^AC
Alw44I 155 GTGCAC G^TGCAC
AlwNI 862 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 155 GTGCAC G^TGCAC
ApoI 956 GAATTC [GA]^AATT[CT]
AvrII 993 CCTAGG C^CTAGG
BalI 117 TGGCCA TGG^CCA
BbsI 651 GAAGAC GAAGAC(2/6)
BclI 633 TGATCA T^GATCA
BlnI 993 CCTAGG C^CTAGG
BlpI 838 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 838 GCTGAGC GC^T[ACGT]AGC
BpuAI 651 GAAGAC GAAGAC(2/6)
BsaWI 137 TCCGGA [AT]^CCGG[AT]
BseAI 137 TCCGGA T^CCGGA
BsgI 619 GTGCAG GTGCAG(16/14)
BspEI 137 TCCGGA T^CCGGA
BssSI 406 CTCGTG CACGAG(-5/-1)
BstAPI 998 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 420 CGCG CG^CG
BstXI 387 CCACTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 838 GCTGAGC GC^T[ACGT]AGC
DpnII 634 GATC ^GATC
DsaI 419 CCGCGG C^C[GA][CT]GG
EarI 187 GAAGAG CTCTTC(1/4)
EcoRI 956 GAATTC G^AATTC
FauI 857 CCCGC CCCGC(4/6)
FbaI 633 TGATCA T^GATCA
Ksp632I 187 GAAGAG CTCTTC(1/4)
KspI 419 CCGCGG CCGC^GG
MboI 634 GATC ^GATC
MluNI 117 TGGCCA TGG^CCA
MroI 137 TCCGGA T^CCGGA
MscI 117 TGGCCA TGG^CCA
MvnI 420 CGCG CG^CG
NdeII 634 GATC ^GATC
NspI 398 GCATGC [GA]CATG^[CT]
PaeR7I 294 CTCGAG C^TCGAG
PflMI 861 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 231 GTAC GT^AC
SacI 106 GAGCTC GAGCT^C
SacII 419 CCGCGG CCGC^GG
SapI 187 GAAGAGC GCTCTTC(1/4)
Sau3AI 634 GATC ^GATC
SmaI 24 CCCGGG CCC^GGG
SmlI 294 CTCGAG C^T[CT][GA]AG
SphI 398 GCATGC GCATG^C
StuI 298 AGGCCT AGG^CCT
TfiI 579 GATTC G^A[AT]TC
Van91I 861 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 294 CTCGAG C^TCGAG
XmaCI 24 CCCGGG C^CCGGG
XmaI 24 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 106 GAGCTC G[AT]GC[AT]^C
155 GTGCAC
AvaI 24 CCCGGG C^[CT]CG[GA]G
294 CTCGAG
BcgI 296 CGAGGCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
398 GCATGCTGCTCG
BglI 86 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
300 GCCTATGCGGC
BseRI 51 CTCCTC GAGGAG(10/8)
761 CTCCTC
BsiHKAI 106 GAGCTC G[AT]GC[AT]^C
155 GTGCAC
BsmAI 525 GAGAC GTCTC(1/5)
709 GAGAC
BsmFI 931 GGGAC GGGAC(10/14)
217 GTCCC
BsoBI 24 CCCGGG C^[CT]CG[GA]G
294 CTCGAG
BsrDI 41 CATTGC GCAATG(2/0)
925 GCAATG
DraIII 529 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
999 CACAGGGTG
EaeI 117 TGGCCA [CT]^GGCC[GA]
416 TGGCCG
EcoT14I 377 CCTTGG C^C[AT][AT]GG
993 CCTAGG
PleI 438 GACTC GAGTC(4/5)
664 GAGTC
PpuMI 122 AGGTCCT [GA]G^G[AT]CC[CT]
215 AGGTCCC
PvuII 503 CAGCTG CAG^CTG
862 CAGCTG
StyI 377 CCTTGG C^C[AT][AT]GG
993 CCTAGG
Tsp509I 778 AATT ^AATT
957 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AocI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
Cfr13I DdeI DraII Eco81I EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I