Restriction map for
pF1KE0016
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AocI Aor51HI AscI
Asp700I Asp718I AspEI AvrII BanI
BbeI BciVI BfrI BlnI BlpI
Bpu1102I BsaI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HaeII KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tsp45I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 370 GACGTC GACGT^C
AccI 218 GTAGAC GT^[AC][GT]AC
AcsI 721 AAATTC [GA]^AATT[CT]
Alw44I 481 GTGCAC G^TGCAC
AlwNI 1050 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 879 GGGCCC GGGCC^C
ApaLI 481 GTGCAC G^TGCAC
ApoI 721 AAATTC [GA]^AATT[CT]
AseI 1938 ATTAAT AT^TAAT
AsnI 1938 ATTAAT AT^TAAT
AspI 121 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1376 TGCGCA TGC^GCA
BamHI 1145 GGATCC G^GATCC
BbvCI 1603 GCTGAGG CCTCAGC(-5/-2)
BcgI 489 CGAGTGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1950 TGATCA T^GATCA
BglII 1785 AGATCT A^GATCT
BsaWI 1718 ACCGGA [AT]^CCGG[AT]
BsiEI 510 CGGCCG CG[GA][CT]^CG
BsmAI 119 GAGAC GTCTC(1/5)
BspHI 201 TCATGA T^CATGA
BsrDI 1323 CATTGC GCAATG(2/0)
DraI 687 TTTAAA TTT^AAA
EagI 510 CGGCCG C^GGCCG
EclXI 510 CGGCCG C^GGCCG
Eco52I 510 CGGCCG C^GGCCG
FbaI 1950 TGATCA T^GATCA
FspI 1376 TGCGCA TGC^GCA
HindIII 2015 AAGCTT A^AGCTT
HpaI 151 GTTAAC GTT^AAC
NaeI 885 GCCGGC GCC^GGC
NcoI 2064 CCATGG C^CATGG
NgoMIV 885 GCCGGC G^CCGGC
PflFI 121 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 136 GACTC GAGTC(4/5)
PshBI 1938 ATTAAT AT^TAAT
PstI 397 CTGCAG CTGCA^G
PvuII 1496 CAGCTG CAG^CTG
RcaI 201 TCATGA T^CATGA
SmlI 2026 CTCAAG C^T[CT][GA]AG
TaqI 1883 TCGA T^CGA
Tth111I 121 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1883 TCGA T^CGA
XbaI 2103 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 456 CGCG CG^CG
1191 CGCG
AvaI 294 CTCGGG C^[CT]CG[GA]G
1768 CTCGGG
AvaII 162 GGACC G^G[AT]CC
189 GGACC
BalI 647 TGGCCA TGG^CCA
2111 TGGCCA
BanII 879 GGGCCC G[GA]GC[CT]^C
1289 GAGCCC
BbrPI 322 CACGTG CAC^GTG
1798 CACGTG
BbsI 1126 GAAGAC GAAGAC(2/6)
373 GTCTTC
BcnI 909 CCCGG CC^[GC]GG
1515 CCGGG
BglI 433 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1091 GCCTAATTGGC
BpuAI 1126 GAAGAC GAAGAC(2/6)
373 GTCTTC
BsaBI 19 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1786 GATCTCAATC
BsoBI 294 CTCGGG C^[CT]CG[GA]G
1768 CTCGGG
BsrFI 251 ACCGGC [GA]^CCGG[CT]
885 GCCGGC
BstUI 456 CGCG CG^CG
1191 CGCG
BstYI 1145 GGATCC [GA]^GATC[CT]
1785 AGATCT
Cfr10I 251 ACCGGC [GA]^CCGG[CT]
885 GCCGGC
DraIII 307 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
486 CACCGAGTG
EarI 808 CTCTTC CTCTTC(1/4)
1922 GAAGAG
EcoT14I 555 CCTTGG C^C[AT][AT]GG
2064 CCATGG
FauI 882 CCCGC CCCGC(4/6)
1189 CCCGC
HgaI 442 GCGTC GACGC(5/10)
1773 GCGTC
HincII 151 GTTAAC GT[CT]^[GA]AC
328 GTCAAC
HindII 151 GTTAAC GT[CT]^[GA]AC
328 GTCAAC
Ksp632I 808 CTCTTC CTCTTC(1/4)
1922 GAAGAG
MamI 19 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1786 GATCTCAATC
MflI 1145 GGATCC [GA]^GATC[CT]
1785 AGATCT
MluNI 647 TGGCCA TGG^CCA
2111 TGGCCA
MscI 647 TGGCCA TGG^CCA
2111 TGGCCA
MvnI 456 CGCG CG^CG
1191 CGCG
NciI 909 CCCGG CC^[GC]GG
1515 CCGGG
NspI 785 GCATGC [GA]CATG^[CT]
929 GCATGC
PflMI 306 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
608 CCACCTTCTGG
PmaCI 322 CACGTG CAC^GTG
1798 CACGTG
PmlI 322 CACGTG CAC^GTG
1798 CACGTG
PpuMI 161 AGGACCT [GA]G^G[AT]CC[CT]
188 GGGACCT
SfcI 397 CTGCAG C^T[GA][CT]AG
1594 CTACAG
SphI 785 GCATGC GCATG^C
929 GCATGC
StyI 555 CCTTGG C^C[AT][AT]GG
2064 CCATGG
Van91I 306 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
608 CCACCTTCTGG
XhoII 1145 GGATCC [GA]^GATC[CT]
1785 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI BbvI BfaI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI