Restriction map for
pF1KE0079
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApaI ApoI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MflI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 350 GGTACC G^GTACC
AccI 1645 GTCTAC GT^[AC][GT]AC
Alw44I 339 GTGCAC G^TGCAC
AlwI 746 GGATC GGATC(4/5)
AocI 788 CCTCAGG CC^T[ACGT]AGG
ApaLI 339 GTGCAC G^TGCAC
AscI 1725 GGCGCGCC GG^CGCGCC
Asp718I 350 GGTACC G^GTACC
BcgI 1814 CGAGGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 599 CTAG C^TAG
BlpI 1508 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1508 GCTCAGC GC^T[ACGT]AGC
BsrDI 1288 CATTGC GCAATG(2/0)
BssSI 1378 CTCGTG CACGAG(-5/-1)
Bsu36I 788 CCTCAGG CC^T[ACGT]AGG
CelII 1508 GCTCAGC GC^T[ACGT]AGC
EarI 655 CTCTTC CTCTTC(1/4)
Eco81I 788 CCTCAGG CC^T[ACGT]AGG
HgaI 661 GCGTC GACGC(5/10)
KpnI 350 GGTACC GGTAC^C
Ksp632I 655 CTCTTC CTCTTC(1/4)
MaeI 599 CTAG C^TAG
MslI 1635 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 307 GCCGGC GCC^GGC
NcoI 1694 CCATGG C^CATGG
NgoMIV 307 GCCGGC G^CCGGC
PaeR7I 1812 CTCGAG C^TCGAG
PflMI 1390 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 70 CTGCAG CTGCA^G
SfiI 288 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1812 CTCGAG C^T[CT][GA]AG
StuI 1808 AGGCCT AGG^CCT
TaqI 1813 TCGA T^CGA
TthHB8I 1813 TCGA T^CGA
Van91I 1390 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1812 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 20 GGCGCC G[GA]^CG[CT]C
412 GGCGCC
AflIII 164 ACATGT A^C[GA][CT]GT
931 ACATGT
AlwNI 507 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1075 CAGTTACTG
AspHI 339 GTGCAC G[AT]GC[AT]^C
1182 GAGCAC
BalI 1284 TGGCCA TGG^CCA
1548 TGGCCA
BanII 1216 GAGCCC G[GA]GC[CT]^C
1690 GAGCCC
BbeI 20 GGCGCC GGCGC^C
412 GGCGCC
BsaHI 20 GGCGCC G[GA]^CG[CT]C
412 GGCGCC
BsaI 1469 GAGACC GGTCTC(1/5)
1717 GAGACC
BsiHKAI 339 GTGCAC G[AT]GC[AT]^C
1182 GAGCAC
BspLU11I 164 ACATGT A^CATGT
931 ACATGT
BsrFI 307 GCCGGC [GA]^CCGG[CT]
1818 GCCGGT
Cfr10I 307 GCCGGC [GA]^CCGG[CT]
1818 GCCGGT
DraII 287 AGGCCCT [GA]G^G[ACGT]CC[CT]
1054 AGGCCCT
DraIII 390 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
444 CACAGTGTG
DrdI 796 GACAGTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1239 GACTCCCATGTC
EcoO109I 287 AGGCCCT [GA]G^G[ACGT]CC[CT]
1054 AGGCCCT
Hin1I 20 GGCGCC G[GA]^CG[CT]C
412 GGCGCC
HphI 793 GGTGA GGTGA(8/7)
1614 GGTGA
KasI 20 GGCGCC G^GCGCC
412 GGCGCC
MluNI 1284 TGGCCA TGG^CCA
1548 TGGCCA
MscI 1284 TGGCCA TGG^CCA
1548 TGGCCA
MseI 640 TTAA T^TAA
1156 TTAA
MspA1I 28 CCGCTG C[AC]G^C[GT]G
1605 CAGCGG
NarI 20 GGCGCC GG^CGCC
412 GGCGCC
SfcI 70 CTGCAG C^T[GA][CT]AG
702 CTATAG
SfoI 20 GGCGCC GGC^GCC
412 GGCGCC
Tru9I 640 TTAA T^TAA
1156 TTAA
Tsp45I 794 GTGAC ^GT[GC]AC
1573 GTGAC
Tsp509I 645 AATT ^AATT
733 AATT
XcmI 1410 CCAGTGCGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1473 CCAGCCCGAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanI BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI