Restriction map for
pF1KE0039
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FseI FspI Hin1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 1290 GTAC GT^AC
AflIII 1113 ACGTGT A^C[GA][CT]GT
AlwNI 218 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 790 CCTAAGG CC^T[ACGT]AGG
AseI 948 ATTAAT AT^TAAT
AsnI 948 ATTAAT AT^TAAT
AvaI 485 CTCGAG C^[CT]CG[GA]G
BamHI 723 GGATCC G^GATCC
BanII 667 GGGCTC G[GA]GC[CT]^C
BbsI 258 GAAGAC GAAGAC(2/6)
BciVI 1265 GTATCC GTATCC(6/5)
BcnI 559 CCGGG CC^[GC]GG
BglI 290 GCCAGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 802 GCTAAGC GC^T[ACGT]AGC
BmyI 667 GGGCTC G[AGT]GC[ACT]^C
BpmI 1188 CTGGAG CTGGAG(16/14)
Bpu1102I 802 GCTAAGC GC^T[ACGT]AGC
BpuAI 258 GAAGAC GAAGAC(2/6)
BseRI 1410 CTCCTC GAGGAG(10/8)
BsmFI 521 GTCCC GGGAC(10/14)
BsoBI 485 CTCGAG C^[CT]CG[GA]G
Bsp1286I 667 GGGCTC G[AGT]GC[ACT]^C
BspMI 798 ACCTGC ACCTGC(4/8)
BssSI 368 CACGAG CACGAG(-5/-1)
Bsu36I 790 CCTAAGG CC^T[ACGT]AGG
CelII 802 GCTAAGC GC^T[ACGT]AGC
CfoI 6 GCGC GCG^C
Eco81I 790 CCTAAGG CC^T[ACGT]AGG
FauI 1230 CCCGC CCCGC(4/6)
HaeII 5 AGCGCC [GA]GCGC^[CT]
HgaI 856 GACGC GACGC(5/10)
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
HindIII 1485 AAGCTT A^AGCTT
MaeII 1113 ACGT A^CGT
MspA1I 977 CAGCTG C[AC]G^C[GT]G
NciI 559 CCGGG CC^[GC]GG
PaeR7I 485 CTCGAG C^TCGAG
PflMI 1209 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 483 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 948 ATTAAT AT^TAAT
PvuII 977 CAGCTG CAG^CTG
RsaI 1290 GTAC GT^AC
SfcI 336 CTACAG C^T[GA][CT]AG
SspI 1354 AATATT AAT^ATT
TaqI 486 TCGA T^CGA
TthHB8I 486 TCGA T^CGA
Van91I 1209 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 768 CCAGTCCATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 485 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 62 TCCGGA T^CCGGA
357 TCCGGA
AciI 873 CCGC CCGC(-3/-1)
1231 CCGC
BalI 537 TGGCCA TGG^CCA
964 TGGCCA
BanI 276 GGTGCC G^G[CT][GA]CC
902 GGCACC
BclI 684 TGATCA T^GATCA
1049 TGATCA
BsaWI 62 TCCGGA [AT]^CCGG[AT]
357 TCCGGA
BseAI 62 TCCGGA T^CCGGA
357 TCCGGA
BsmAI 170 GTCTC GTCTC(1/5)
715 GTCTC
BspEI 62 TCCGGA T^CCGGA
357 TCCGGA
BstAPI 367 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1178 GCATTTGGTGC
BstYI 723 GGATCC [GA]^GATC[CT]
914 GGATCT
DraI 892 TTTAAA TTT^AAA
1027 TTTAAA
EaeI 537 TGGCCA [CT]^GGCC[GA]
964 TGGCCA
FbaI 684 TGATCA T^GATCA
1049 TGATCA
MflI 723 GGATCC [GA]^GATC[CT]
914 GGATCT
MluNI 537 TGGCCA TGG^CCA
964 TGGCCA
MroI 62 TCCGGA T^CCGGA
357 TCCGGA
MscI 537 TGGCCA TGG^CCA
964 TGGCCA
NcoI 492 CCATGG C^CATGG
1214 CCATGG
SfaNI 1129 GATGC GCATC(5/9)
1276 GATGC
SmlI 247 CTTGAG C^T[CT][GA]AG
485 CTCGAG
TspRI 218 CAGTG CA[GC]TG[ACGT][ACGT]^
662 CACTG
XhoII 723 GGATCC [GA]^GATC[CT]
914 GGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BsaJI BsiYI BslI BspHI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RcaI Sau3AI
Sau96I ScrFI StyI TfiI Tru9I
TseI Tsp45I Tsp509I