Restriction map for
pF1KB6163
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI BbeI BbrPI BciVI
BclI BfrI BsaAI BsaBI BsaI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 1458 GGTACC G^GTACC
AccI 1524 GTCTAC GT^[AC][GT]AC
AccII 1290 CGCG CG^CG
AcyI 1659 GACGCC G[GA]^CG[CT]C
Alw44I 1072 GTGCAC G^TGCAC
ApaLI 1072 GTGCAC G^TGCAC
Asp718I 1458 GGTACC G^GTACC
AspHI 1072 GTGCAC G[AT]GC[AT]^C
AviII 1217 TGCGCA TGC^GCA
AvrII 510 CCTAGG C^CTAGG
BalI 659 TGGCCA TGG^CCA
BamHI 448 GGATCC G^GATCC
BbsI 1336 GTCTTC GAAGAC(2/6)
BcgI 1251 CGACTTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1393 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1166 AGATCT A^GATCT
BlnI 510 CCTAGG C^CTAGG
BlpI 139 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 139 GCTCAGC GC^T[ACGT]AGC
BpuAI 1336 GTCTTC GAAGAC(2/6)
BsaHI 1659 GACGCC G[GA]^CG[CT]C
BsgI 1654 GTGCAG GTGCAG(16/14)
BsiEI 87 CGATCG CG[GA][CT]^CG
BsiHKAI 1072 GTGCAC G[AT]GC[AT]^C
BsmBI 22 GAGACG CGTCTC(1/5)
Bsp1407I 854 TGTACA T^GTACA
BspHI 731 TCATGA T^CATGA
BspMI 1730 ACCTGC ACCTGC(4/8)
BsrDI 1403 CATTGC GCAATG(2/0)
BsrGI 854 TGTACA T^GTACA
BstEII 42 GGTCACC G^GT[ACGT]ACC
BstPI 42 GGTCACC G^GT[ACGT]ACC
BstUI 1290 CGCG CG^CG
BstXI 527 CCAACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 139 GCTCAGC GC^T[ACGT]AGC
DsaI 1703 CCATGG C^C[GA][CT]GG
EcoNI 609 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 42 GGTCACC G^GT[ACGT]ACC
EcoRV 1482 GATATC GAT^ATC
FspI 1217 TGCGCA TGC^GCA
HaeII 1591 AGCGCC [GA]GCGC^[CT]
Hin1I 1659 GACGCC G[GA]^CG[CT]C
KpnI 1458 GGTACC GGTAC^C
MluNI 659 TGGCCA TGG^CCA
MscI 659 TGGCCA TGG^CCA
MseI 134 TTAA T^TAA
MslI 651 CATGATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1290 CGCG CG^CG
NcoI 1703 CCATGG C^CATGG
NheI 1111 GCTAGC G^CTAGC
PflMI 1623 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1363 GAGTC GAGTC(4/5)
PvuI 87 CGATCG CGAT^CG
RcaI 731 TCATGA T^CATGA
SapI 1284 GCTCTTC GCTCTTC(1/4)
SbfI 1731 CCTGCAGG CCTGCA^GG
Sse8387I 1731 CCTGCAGG CCTGCA^GG
SspBI 854 TGTACA T^GTACA
StuI 513 AGGCCT AGG^CCT
Tru9I 134 TTAA T^TAA
Tsp509I 75 AATT ^AATT
Van91I 1623 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1623 CCAGCTTTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 855 GTAC GT^AC
1459 GTAC
ApaI 424 GGGCCC GGGCC^C
800 GGGCCC
BanI 1045 GGCACC G^G[CT][GA]CC
1458 GGTACC
BbvCI 314 CCTCAGC CCTCAGC(-5/-2)
1502 CCTCAGC
BfaI 511 CTAG C^TAG
1112 CTAG
BsaWI 1033 TCCGGT [AT]^CCGG[AT]
1048 ACCGGA
BseRI 1156 CTCCTC GAGGAG(10/8)
408 GAGGAG
BsrI 455 CCAGT ACTGG(1/-1)
1333 CCAGT
CfoI 1218 GCGC GCG^C
1592 GCGC
EaeI 659 TGGCCA [CT]^GGCC[GA]
926 TGGCCG
HhaI 1218 GCGC GCG^C
1592 GCGC
HinP1I 1218 GCGC G^CGC
1592 GCGC
MaeI 511 CTAG C^TAG
1112 CTAG
RsaI 855 GTAC GT^AC
1459 GTAC
SmaI 890 CCCGGG CCC^GGG
1027 CCCGGG
XmaCI 890 CCCGGG C^CCGGG
1027 CCCGGG
XmaI 890 CCCGGG C^CCGGG
1027 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII