Restriction map for
pF1KB9969
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsaI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KpnI MamI MfeI
MluI MseI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 83 TCCGGA T^CCGGA
AflIII 533 ACGTGT A^C[GA][CT]GT
AocI 1219 CCTGAGG CC^T[ACGT]AGG
AvaII 1523 GGTCC G^G[AT]CC
AviII 1028 TGCGCA TGC^GCA
BbsI 1257 GAAGAC GAAGAC(2/6)
BfaI 77 CTAG C^TAG
BpuAI 1257 GAAGAC GAAGAC(2/6)
BsaAI 532 TACGTG [CT]AC^GT[GA]
BsaWI 83 TCCGGA [AT]^CCGG[AT]
BseAI 83 TCCGGA T^CCGGA
BsmBI 218 GAGACG CGTCTC(1/5)
BsmFI 1163 GGGAC GGGAC(10/14)
BspEI 83 TCCGGA T^CCGGA
BspHI 893 TCATGA T^CATGA
BsrDI 1118 GCAATG GCAATG(2/0)
BsrI 123 ACTGG ACTGG(1/-1)
BssHII 714 GCGCGC G^CGCGC
BstEII 1195 GGTGACC G^GT[ACGT]ACC
BstPI 1195 GGTGACC G^GT[ACGT]ACC
BstXI 1152 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1219 CCTGAGG CC^T[ACGT]AGG
Eco81I 1219 CCTGAGG CC^T[ACGT]AGG
EcoNI 1374 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1195 GGTGACC G^GT[ACGT]ACC
FspI 1028 TGCGCA TGC^GCA
HindIII 427 AAGCTT A^AGCTT
KspI 623 CCGCGG CCGC^GG
MaeI 77 CTAG C^TAG
MroI 83 TCCGGA T^CCGGA
NaeI 1105 GCCGGC GCC^GGC
NgoMIV 1105 GCCGGC G^CCGGC
PaeR7I 1089 CTCGAG C^TCGAG
PflMI 1497 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 403 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 893 TCATGA T^CATGA
SacI 1092 GAGCTC GAGCT^C
SacII 623 CCGCGG CCGC^GG
ScaI 467 AGTACT AGT^ACT
SexAI 791 ACCTGGT A^CC[AT]GGT
SmlI 1089 CTCGAG C^T[CT][GA]AG
StuI 683 AGGCCT AGG^CCT
Van91I 1497 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 782 CCAAGTCCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1089 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 468 GTAC GT^AC
489 GTAC
AlwI 255 GATCC GGATC(4/5)
705 GATCC
AlwNI 661 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1498 CAGGGCCTG
BanII 201 GGGCTC G[GA]GC[CT]^C
1092 GAGCTC
BbeI 751 GGCGCC GGCGC^C
1414 GGCGCC
BlpI 415 GCTGAGC GC^T[ACGT]AGC
996 GCTCAGC
Bpu1102I 415 GCTGAGC GC^T[ACGT]AGC
996 GCTCAGC
BsgI 1327 CTGCAC GTGCAG(16/14)
721 GTGCAG
BspMI 127 GCAGGT ACCTGC(4/8)
735 GCAGGT
BstAPI 660 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
723 GCAGCTCATGC
BstYI 254 AGATCC [GA]^GATC[CT]
704 AGATCC
CelII 415 GCTGAGC GC^T[ACGT]AGC
996 GCTCAGC
DraII 1465 AGGCCCT [GA]G^G[ACGT]CC[CT]
1499 AGGGCCT
EcoO109I 1465 AGGCCCT [GA]G^G[ACGT]CC[CT]
1499 AGGGCCT
KasI 751 GGCGCC G^GCGCC
1414 GGCGCC
MflI 254 AGATCC [GA]^GATC[CT]
704 AGATCC
MslI 93 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1621 CACGGGGATG
NarI 751 GGCGCC GG^CGCC
1414 GGCGCC
NcoI 98 CCATGG C^CATGG
1097 CCATGG
PstI 484 CTGCAG CTGCA^G
1339 CTGCAG
RsaI 468 GTAC GT^AC
489 GTAC
SapI 438 GAAGAGC GCTCTTC(1/4)
1350 GAAGAGC
SfaNI 24 GATGC GCATC(5/9)
1266 GATGC
SfoI 751 GGCGCC GGC^GCC
1414 GGCGCC
SmaI 1295 CCCGGG CCC^GGG
1424 CCCGGG
TaqI 494 TCGA T^CGA
1090 TCGA
Tsp509I 109 AATT ^AATT
529 AATT
TthHB8I 494 TCGA T^CGA
1090 TCGA
XhoII 254 AGATCC [GA]^GATC[CT]
704 AGATCC
XmaCI 1295 CCCGGG C^CCGGG
1424 CCCGGG
XmaI 1295 CCCGGG C^CCGGG
1424 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI BalI BanI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfcI
StyI TfiI TseI Tsp45I TspRI