Restriction map for
pF1KB9910
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvrII
BalI BamHI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI HpaI KspI MamI MfeI
MflI MluI MluNI MscI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 1615 GACGTC GACGT^C
AccIII 614 TCCGGA T^CCGGA
AcsI 148 AAATTT [GA]^AATT[CT]
AflIII 707 ACACGT A^C[GA][CT]GT
Aor51HI 797 AGCGCT AGC^GCT
ApoI 148 AAATTT [GA]^AATT[CT]
Asp700I 1171 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 773 TGCGCA TGC^GCA
BbeI 19 GGCGCC GGCGC^C
BbrPI 459 CACGTG CAC^GTG
BbsI 1479 GAAGAC GAAGAC(2/6)
BclI 368 TGATCA T^GATCA
BglI 874 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1479 GAAGAC GAAGAC(2/6)
BseAI 614 TCCGGA T^CCGGA
BsiEI 53 CGGCCG CG[GA][CT]^CG
BsiWI 1082 CGTACG C^GTACG
BsmFI 467 GGGAC GGGAC(10/14)
BsmI 136 GAATGC GAATGC(1/-1)
BspEI 614 TCCGGA T^CCGGA
BspHI 819 TCATGA T^CATGA
DraI 1024 TTTAAA TTT^AAA
DrdI 925 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 696 CCACGG C^C[GA][CT]GG
EagI 53 CGGCCG C^GGCCG
EclXI 53 CGGCCG C^GGCCG
Eco47III 797 AGCGCT AGC^GCT
Eco52I 53 CGGCCG C^GGCCG
EcoNI 863 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 368 TGATCA T^GATCA
FspI 773 TGCGCA TGC^GCA
HincII 1152 GTCAAC GT[CT]^[GA]AC
HindII 1152 GTCAAC GT[CT]^[GA]AC
KasI 19 GGCGCC G^GCGCC
MroI 614 TCCGGA T^CCGGA
NarI 19 GGCGCC GG^CGCC
NspI 580 GCATGC [GA]CATG^[CT]
PaeR7I 603 CTCGAG C^TCGAG
PflMI 1071 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1512 GAGTC GAGTC(4/5)
PmaCI 459 CACGTG CAC^GTG
PmlI 459 CACGTG CAC^GTG
PshAI 823 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 819 TCATGA T^CATGA
SfoI 19 GGCGCC GGC^GCC
SmaI 158 CCCGGG CCC^GGG
SpeI 1218 ACTAGT A^CTAGT
SphI 580 GCATGC GCATG^C
Van91I 1071 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 603 CTCGAG C^TCGAG
XmaCI 158 CCCGGG C^CCGGG
XmaI 158 CCCGGG C^CCGGG
XmnI 1171 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 548 GGTACC G^GTACC
1690 GGTACC
AccII 51 CGCG CG^CG
985 CGCG
AlwI 779 GGATC GGATC(4/5)
1040 GGATC
Asp718I 548 GGTACC G^GTACC
1690 GGTACC
BsaAI 459 CACGTG [CT]AC^GT[GA]
708 CACGTA
BsaWI 614 TCCGGA [AT]^CCGG[AT]
969 TCCGGT
BsrDI 1620 CATTGC GCAATG(2/0)
1270 GCAATG
BstUI 51 CGCG CG^CG
985 CGCG
EarI 198 GAAGAG CTCTTC(1/4)
357 GAAGAG
EcoT14I 845 CCTTGG C^C[AT][AT]GG
1312 CCAAGG
HaeII 19 GGCGCC [GA]GCGC^[CT]
797 AGCGCT
HgaI 541 GACGC GACGC(5/10)
823 GACGC
HindIII 529 AAGCTT A^AGCTT
1423 AAGCTT
KpnI 548 GGTACC GGTAC^C
1690 GGTACC
Ksp632I 198 GAAGAG CTCTTC(1/4)
357 GAAGAG
MvnI 51 CGCG CG^CG
985 CGCG
PpuMI 467 GGGACCT [GA]G^G[AT]CC[CT]
1403 AGGTCCT
PstI 1327 CTGCAG CTGCA^G
1408 CTGCAG
SfcI 1327 CTGCAG C^T[GA][CT]AG
1408 CTGCAG
SmlI 603 CTCGAG C^T[CT][GA]AG
1468 CTTGAG
StyI 845 CCTTGG C^C[AT][AT]GG
1312 CCAAGG
TfiI 97 GATTC G^A[AT]TC
397 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I