Restriction map for
pF1KB6983
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseRI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
MaeI MamI MfeI MluI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 569 TCCGGA T^CCGGA
AfaI 288 GTAC GT^AC
AlwNI 373 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 58 GGGCCC GGGCC^C
AseI 328 ATTAAT AT^TAAT
AsnI 328 ATTAAT AT^TAAT
AspHI 589 GAGCTC G[AT]GC[AT]^C
BalI 300 TGGCCA TGG^CCA
BanI 481 GGCGCC G^G[CT][GA]CC
BbeI 481 GGCGCC GGCGC^C
BbrPI 493 CACGTG CAC^GTG
BpmI 436 CTGGAG CTGGAG(16/14)
BsaAI 493 CACGTG [CT]AC^GT[GA]
BsaWI 569 TCCGGA [AT]^CCGG[AT]
BseAI 569 TCCGGA T^CCGGA
BsiHKAI 589 GAGCTC G[AT]GC[AT]^C
BsmBI 526 CGTCTC CGTCTC(1/5)
BspEI 569 TCCGGA T^CCGGA
BsrFI 335 ACCGGC [GA]^CCGG[CT]
BssHII 128 GCGCGC G^CGCGC
Cfr10I 335 ACCGGC [GA]^CCGG[CT]
DraIII 490 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
KasI 481 GGCGCC G^GCGCC
KspI 251 CCGCGG CCGC^GG
MaeIII 441 GTCAC ^GT[ACGT]AC
MluNI 300 TGGCCA TGG^CCA
MroI 569 TCCGGA T^CCGGA
MscI 300 TGGCCA TGG^CCA
MseI 329 TTAA T^TAA
NarI 481 GGCGCC GG^CGCC
NspI 290 ACATGC [GA]CATG^[CT]
PflMI 489 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 493 CACGTG CAC^GTG
PmlI 493 CACGTG CAC^GTG
PshBI 328 ATTAAT AT^TAAT
PstI 207 CTGCAG CTGCA^G
PvuII 553 CAGCTG CAG^CTG
RsaI 288 GTAC GT^AC
SacI 589 GAGCTC GAGCT^C
SacII 251 CCGCGG CCGC^GG
SapI 363 GCTCTTC GCTCTTC(1/4)
SfaNI 221 GCATC GCATC(5/9)
SfcI 207 CTGCAG C^T[GA][CT]AG
SfoI 481 GGCGCC GGC^GCC
StuI 509 AGGCCT AGG^CCT
TfiI 375 GATTC G^A[AT]TC
Tru9I 329 TTAA T^TAA
Tsp45I 441 GTCAC ^GT[GC]AC
Van91I 489 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 15 GGCGTC G[GA]^CG[CT]C
481 GGCGCC
AvaI 41 CCCGGG C^[CT]CG[GA]G
61 CCCGGG
BglII 203 AGATCT A^GATCT
432 AGATCT
BsaHI 15 GGCGTC G[GA]^CG[CT]C
481 GGCGCC
BsmAI 82 GAGAC GTCTC(1/5)
527 GTCTC
BsoBI 41 CCCGGG C^[CT]CG[GA]G
61 CCCGGG
BstYI 203 AGATCT [GA]^GATC[CT]
432 AGATCT
DsaI 251 CCGCGG C^C[GA][CT]GG
303 CCACGG
EaeI 300 TGGCCA [CT]^GGCC[GA]
515 CGGCCA
EarI 364 CTCTTC CTCTTC(1/4)
262 GAAGAG
HaeII 446 AGCGCC [GA]GCGC^[CT]
481 GGCGCC
Hin1I 15 GGCGTC G[GA]^CG[CT]C
481 GGCGCC
Ksp632I 364 CTCTTC CTCTTC(1/4)
262 GAAGAG
MaeII 494 ACGT A^CGT
533 ACGT
MflI 203 AGATCT [GA]^GATC[CT]
432 AGATCT
NlaIII 276 CATG CATG^
291 CATG
PleI 190 GACTC GAGTC(4/5)
439 GAGTC
SmaI 41 CCCGGG CCC^GGG
61 CCCGGG
TspRI 210 CAGTG CA[GC]TG[ACGT][ACGT]^
609 CAGTG
XhoII 203 AGATCT [GA]^GATC[CT]
432 AGATCT
XmaCI 41 CCCGGG C^CCGGG
61 CCCGGG
XmaI 41 CCCGGG C^CCGGG
61 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV PpuMI Sau3AI
Sau96I ScrFI TseI