##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BanI        BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaJI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TseI        Tsp45I      
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI          194  GAATTC               [GA]^AATT[CT]
AlwNI          89  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          194  GAATTC               [GA]^AATT[CT]
Asp700I       113  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         528  GGATCC               G^GATCC
BanII          33  GAGCCC               G[GA]GC[CT]^C
BcnI          301  CCGGG                CC^[GC]GG
BmyI           33  GAGCCC               G[AGT]GC[ACT]^C
BsaI           74  GAGACC               GGTCTC(1/5)
BseRI         230  CTCCTC               GAGGAG(10/8)
BsmAI          74  GAGAC                GTCTC(1/5)
BsmFI         461  GTCCC                GGGAC(10/14)
Bsp1286I       33  GAGCCC               G[AGT]GC[ACT]^C
BsrBI          83  CCGCTC               CCGCTC(-3/-3)
BssKI         301  CCGGG                ^CC[ACGT]GG
BstF5I        441  CATCC                GGATG(2/0)
BstYI         528  GGATCC               [GA]^GATC[CT]
Cac8I         207  GCTAGC               GC[ACGT]^[ACGT]GC
EcoRI         194  GAATTC               G^AATTC
EcoRV         337  GATATC               GAT^ATC
FauI          463  CCCGC                CCCGC(4/6)
Fnu4HI         82  GCCGC                GC^[ACGT]GC
FokI          441  CATCC                GGATG(9/13)
HaeIII        251  GGCC                 GG^CC
HapII         301  CCGG                 C^CGG
HpaII         301  CCGG                 C^CGG
ItaI           82  GCCGC                GC^[ACGT]GC
MaeIII        379  GTTAC                ^GT[ACGT]AC
MflI          528  GGATCC               [GA]^GATC[CT]
MspI          301  CCGG                 C^CGG
NciI          301  CCGGG                CC^[GC]GG
NheI          207  GCTAGC               G^CTAGC
NlaIV         528  GGATCC               GG[ACGT]^[ACGT]CC
PflMI         593  CCAGACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScrFI         301  CCGGG                CC^[ACGT]GG
SfaNI          61  GATGC                GCATC(5/9)
SpeI          181  ACTAGT               A^CTAGT
TfiI          395  GAATC                G^A[AT]TC
Van91I        593  CCAGACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         528  GGATCC               [GA]^GATC[CT]
XmnI          113  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AluI          206  AGCT                 AG^CT
              210  AGCT                
BpmI           86  CTCCAG               CTGGAG(16/14)
              456  CTGGAG              
BsrI          455  ACTGG                ACTGG(1/-1)
                5  CCAGT               
DdeI          170  CTAAG                C^T[ACGT]AG
              554  CTCAG               
MboII          97  GAAGA                GAAGA(8/7)
              135  GAAGA               
MseI          365  TTAA                 T^TAA
              446  TTAA                
PleI          425  GAGTC                GAGTC(4/5)
              459  GAGTC               
Tru9I         365  TTAA                 T^TAA
              446  TTAA                
Tsp509I       195  AATT                 ^AATT
              238  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AciI        AlwI        AvaII       BfaI        BsiYI       
BslI        Cfr13I      DpnII       HinfI       MaeI        
MboI        MnlI        NdeII       Sau3AI      Sau96I      
TspRI