Restriction map for
pF1KB6917
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DrdI
Eam1105I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HindIII HpaI HphI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfcI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 223 GTCGAC GT^[AC][GT]AC
AlwI 291 GGATC GGATC(4/5)
AlwNI 502 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 578 AGCGCT AGC^GCT
Asp700I 39 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 485 CCCGGG C^[CT]CG[GA]G
BbsI 439 GAAGAC GAAGAC(2/6)
BglI 133 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 439 GAAGAC GAAGAC(2/6)
BseRI 427 GAGGAG GAGGAG(10/8)
BsmAI 125 GAGAC GTCTC(1/5)
BsmBI 125 GAGACG CGTCTC(1/5)
BsmFI 260 GGGAC GGGAC(10/14)
BsoBI 485 CCCGGG C^[CT]CG[GA]G
BsrBI 16 GAGCGG CCGCTC(-3/-3)
DpnII 292 GATC ^GATC
DraIII 498 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 30 CCACGG C^C[GA][CT]GG
EarI 43 CTCTTC CTCTTC(1/4)
Eco47III 578 AGCGCT AGC^GCT
EcoNI 194 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 223 GTCGAC GT[CT]^[GA]AC
HindII 223 GTCGAC GT[CT]^[GA]AC
HinfI 172 GATTC G^A[ACGT]TC
Ksp632I 43 CTCTTC CTCTTC(1/4)
MaeII 235 ACGT A^CGT
MaeIII 611 GTGAC ^GT[ACGT]AC
MboI 292 GATC ^GATC
MspA1I 315 CAGCTG C[AC]G^C[GT]G
NdeII 292 GATC ^GATC
NotI 130 GCGGCCGC GC^GGCCGC
NruI 294 TCGCGA TCG^CGA
NspI 490 GCATGC [GA]CATG^[CT]
PflMI 330 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 315 CAGCTG CAG^CTG
SalI 223 GTCGAC G^TCGAC
Sau3AI 292 GATC ^GATC
SfaNI 240 GATGC GCATC(5/9)
SfiI 132 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 485 CCCGGG CCC^GGG
SphI 490 GCATGC GCATG^C
TfiI 172 GATTC G^A[AT]TC
Tsp45I 611 GTGAC ^GT[GC]AC
TspRI 502 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 330 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 485 CCCGGG C^CCGGG
XmaI 485 CCCGGG C^CCGGG
XmnI 39 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 316 AGCT AG^CT
539 AGCT
BanI 6 GGCGCC G^G[CT][GA]CC
159 GGCGCC
BbeI 6 GGCGCC GGCGC^C
159 GGCGCC
BbvI 249 GCAGC GCAGC(8/12)
375 GCAGC
BsgI 517 CTGCAC GTGCAG(16/14)
459 GTGCAG
BspMI 356 ACCTGC ACCTGC(4/8)
515 ACCTGC
BsrI 303 ACTGG ACTGG(1/-1)
390 CCAGT
BssHII 62 GCGCGC G^CGCGC
64 GCGCGC
DdeI 410 CTAAG C^T[ACGT]AG
445 CTAAG
EagI 131 CGGCCG C^GGCCG
205 CGGCCG
EclXI 131 CGGCCG C^GGCCG
205 CGGCCG
Eco52I 131 CGGCCG C^GGCCG
205 CGGCCG
KasI 6 GGCGCC G^GCGCC
159 GGCGCC
NarI 6 GGCGCC GG^CGCC
159 GGCGCC
NlaIII 384 CATG CATG^
491 CATG
SfoI 6 GGCGCC GGC^GCC
159 GGCGCC
TseI 249 GCAGC G^C[AT]GC
375 GCAGC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AcyI BcnI BsaHI
BsaJI BsiEI BsiYI BslI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MboII MnlI MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TaqI TthHB8I