Restriction map for
pF1KB6812
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FauI FbaI Fnu4HI FseI FspI
HgaI HincII HindII HindIII HpaI
ItaI KpnI KspI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I TseI
Tsp45I Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 25 GGCGCC G[GA]^CG[CT]C
AfaI 511 GTAC GT^AC
AflIII 408 ACATGT A^C[GA][CT]GT
AocI 160 CCTGAGG CC^T[ACGT]AGG
AvaI 476 CTCGGG C^[CT]CG[GA]G
AvaII 399 GGACC G^G[AT]CC
BbeI 25 GGCGCC GGCGC^C
BfaI 55 CTAG C^TAG
BpmI 437 CTGGAG CTGGAG(16/14)
BsaHI 25 GGCGCC G[GA]^CG[CT]C
BsmAI 62 GAGAC GTCTC(1/5)
BsmFI 111 GGGAC GGGAC(10/14)
BsoBI 476 CTCGGG C^[CT]CG[GA]G
BspLU11I 408 ACATGT A^CATGT
BstAPI 538 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 225 AGATCC [GA]^GATC[CT]
Bsu36I 160 CCTGAGG CC^T[ACGT]AGG
EarI 166 GAAGAG CTCTTC(1/4)
Eco81I 160 CCTGAGG CC^T[ACGT]AGG
EcoRV 483 GATATC GAT^ATC
HaeII 25 GGCGCC [GA]GCGC^[CT]
Hin1I 25 GGCGCC G[GA]^CG[CT]C
HinfI 296 GAATC G^A[ACGT]TC
HphI 420 GGTGA GGTGA(8/7)
KasI 25 GGCGCC G^GCGCC
Ksp632I 166 GAAGAG CTCTTC(1/4)
MaeI 55 CTAG C^TAG
MflI 225 AGATCC [GA]^GATC[CT]
MspA1I 194 CAGCTG C[AC]G^C[GT]G
NarI 25 GGCGCC GG^CGCC
NspI 408 ACATGT [GA]CATG^[CT]
PflMI 300 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 194 CAGCTG CAG^CTG
RsaI 511 GTAC GT^AC
SapI 166 GAAGAGC GCTCTTC(1/4)
SfcI 365 CTACAG C^T[GA][CT]AG
SfoI 25 GGCGCC GGC^GCC
TaqI 294 TCGA T^CGA
TfiI 296 GAATC G^A[AT]TC
TthHB8I 294 TCGA T^CGA
Van91I 300 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 225 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 226 GATCC GGATC(4/5)
291 GGATC
AlwNI 211 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
301 CAGGCCCTG
BanI 25 GGCGCC G^G[CT][GA]CC
252 GGCACC
BcnI 35 CCCGG CC^[GC]GG
530 CCCGG
CfoI 26 GCGC GCG^C
454 GCGC
DpnII 226 GATC ^GATC
292 GATC
DsaI 68 CCATGG C^C[GA][CT]GG
395 CCATGG
EcoNI 124 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
451 CCTGCGCCAGG
EcoT14I 68 CCATGG C^C[AT][AT]GG
395 CCATGG
HapII 36 CCGG C^CGG
531 CCGG
HhaI 26 GCGC GCG^C
454 GCGC
HinP1I 26 GCGC G^CGC
454 GCGC
HpaII 36 CCGG C^CGG
531 CCGG
MboI 226 GATC ^GATC
292 GATC
MspI 36 CCGG C^CGG
531 CCGG
NciI 35 CCCGG CC^[GC]GG
530 CCCGG
NcoI 68 CCATGG C^CATGG
395 CCATGG
NdeII 226 GATC ^GATC
292 GATC
Sau3AI 226 GATC ^GATC
292 GATC
StyI 68 CCATGG C^C[AT][AT]GG
395 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI ApaI BanII BglI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
FokI HaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI TspRI