Restriction map for
pF1KB6809
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BamHI BbrPI BcgI BciVI
BclI BfrI BglII BpmI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeII MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 316 GTCTAC GT^[AC][GT]AC
AflIII 323 ACATGT A^C[GA][CT]GT
Alw44I 208 GTGCAC G^TGCAC
AlwI 430 GATCC GGATC(4/5)
ApaLI 208 GTGCAC G^TGCAC
AvaI 467 CCCGAG C^[CT]CG[GA]G
AvrII 36 CCTAGG C^CTAGG
BanI 420 GGCGCC G^G[CT][GA]CC
BanII 346 GAGCTC G[GA]GC[CT]^C
BbeI 420 GGCGCC GGCGC^C
BbsI 375 GTCTTC GAAGAC(2/6)
BbvCI 279 CCTCAGC CCTCAGC(-5/-2)
BcnI 330 CCGGG CC^[GC]GG
BfaI 37 CTAG C^TAG
BglI 254 GCCGCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 36 CCTAGG C^CTAGG
BlpI 136 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 136 GCTCAGC GC^T[ACGT]AGC
BpuAI 375 GTCTTC GAAGAC(2/6)
BsoBI 467 CCCGAG C^[CT]CG[GA]G
BspLU11I 323 ACATGT A^CATGT
BsrBI 298 CCGCTC CCGCTC(-3/-3)
BstXI 191 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 429 AGATCC [GA]^GATC[CT]
CelII 136 GCTCAGC GC^T[ACGT]AGC
DpnII 430 GATC ^GATC
DsaI 229 CCGCGG C^C[GA][CT]GG
HaeII 420 GGCGCC [GA]GCGC^[CT]
HapII 330 CCGG C^CGG
HgaI 154 GACGC GACGC(5/10)
HpaII 330 CCGG C^CGG
KasI 420 GGCGCC G^GCGCC
KspI 229 CCGCGG CCGC^GG
MaeI 37 CTAG C^TAG
MaeIII 41 GTCAC ^GT[ACGT]AC
MboI 430 GATC ^GATC
MflI 429 AGATCC [GA]^GATC[CT]
MslI 159 CATAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 330 CCGG C^CGG
NarI 420 GGCGCC GG^CGCC
NciI 330 CCGGG CC^[GC]GG
NdeII 430 GATC ^GATC
SacI 346 GAGCTC GAGCT^C
SacII 229 CCGCGG CCGC^GG
Sau3AI 430 GATC ^GATC
SfoI 420 GGCGCC GGC^GCC
SmlI 292 CTCAAG C^T[CT][GA]AG
TaqI 152 TCGA T^CGA
TfiI 30 GATTC G^A[AT]TC
Tsp45I 41 GTCAC ^GT[GC]AC
TthHB8I 152 TCGA T^CGA
XhoII 429 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 230 CGCG CG^CG
337 CGCG
AcyI 154 GACGCC G[GA]^CG[CT]C
420 GGCGCC
AfaI 327 GTAC GT^AC
474 GTAC
AspHI 208 GTGCAC G[AT]GC[AT]^C
346 GAGCTC
AvaII 354 GGTCC G^G[AT]CC
384 GGACC
BalI 185 TGGCCA TGG^CCA
272 TGGCCA
BmyI 208 GTGCAC G[AGT]GC[ACT]^C
346 GAGCTC
BsaHI 154 GACGCC G[GA]^CG[CT]C
420 GGCGCC
BsiHKAI 208 GTGCAC G[AT]GC[AT]^C
346 GAGCTC
BsiYI 86 CCTATGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
111 CCTGGCCTTGG
BslI 86 CCTATGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
111 CCTGGCCTTGG
BsmFI 143 GGGAC GGGAC(10/14)
442 GTCCC
Bsp1286I 208 GTGCAC G[AGT]GC[ACT]^C
346 GAGCTC
BsrI 44 ACTGG ACTGG(1/-1)
361 ACTGG
BstF5I 108 CATCC GGATG(2/0)
276 CATCC
BstUI 230 CGCG CG^CG
337 CGCG
CfoI 336 GCGC GCG^C
421 GCGC
EaeI 185 TGGCCA [CT]^GGCC[GA]
272 TGGCCA
FokI 108 CATCC GGATG(9/13)
276 CATCC
HhaI 336 GCGC GCG^C
421 GCGC
Hin1I 154 GACGCC G[GA]^CG[CT]C
420 GGCGCC
HinP1I 336 GCGC G^CGC
421 GCGC
MluNI 185 TGGCCA TGG^CCA
272 TGGCCA
MscI 185 TGGCCA TGG^CCA
272 TGGCCA
MspA1I 139 CAGCGG C[AC]G^C[GT]G
229 CCGCGG
MvnI 230 CGCG CG^CG
337 CGCG
NlaIV 82 GGCTCC GG[ACGT]^[ACGT]CC
420 GGCGCC
NspI 164 GCATGT [GA]CATG^[CT]
323 ACATGT
RsaI 327 GTAC GT^AC
474 GTAC
SfaNI 463 GATGC GCATC(5/9)
218 GCATC
TspRI 43 CACTG CA[GC]TG[ACGT][ACGT]^
360 CACTG
XcmI 107 CCATCCTGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
188 CCACCATCTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
EcoT14I FauI Fnu4HI HaeIII HinfI
HphI ItaI MboII MnlI MvaI
MwoI NlaIII PleI Sau96I ScrFI
StyI TseI