Restriction map for
pF1KB6740
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AluI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI KasI KpnI Ksp632I
MaeI MaeIII MamI MfeI MluI
MluNI MroI MscI MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI TseI Tsp45I TspRI Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 159 AAATTT [GA]^AATT[CT]
ApaI 365 GGGCCC GGGCC^C
ApoI 159 AAATTT [GA]^AATT[CT]
AspI 124 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 102 GGATCC G^GATCC
BanII 365 GGGCCC G[GA]GC[CT]^C
BmyI 365 GGGCCC G[AGT]GC[ACT]^C
BsaAI 108 TACGTG [CT]AC^GT[GA]
BseRI 61 GAGGAG GAGGAG(10/8)
BsgI 22 CTGCAC GTGCAG(16/14)
BsmAI 349 GAGAC GTCTC(1/5)
BsmBI 349 GAGACG CGTCTC(1/5)
Bsp1286I 365 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 134 TGTACA T^GTACA
BspHI 269 TCATGA T^CATGA
BsrGI 134 TGTACA T^GTACA
BstYI 102 GGATCC [GA]^GATC[CT]
DrdI 67 GACCGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 90 CGGCCA [CT]^GGCC[GA]
EcoNI 54 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII 109 ACGT A^CGT
MflI 102 GGATCC [GA]^GATC[CT]
MslI 114 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 314 CCATGG C^CATGG
NspI 8 ACATGC [GA]CATG^[CT]
PflFI 124 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 128 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 269 TCATGA T^CATGA
SfaNI 140 GCATC GCATC(5/9)
SspBI 134 TGTACA T^GTACA
TaqI 347 TCGA T^CGA
Tsp509I 160 AATT ^AATT
Tth111I 124 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 347 TCGA T^CGA
XhoII 102 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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BsmFI 283 GGGAC GGGAC(10/14)
393 GTCCC
BspMI 20 ACCTGC ACCTGC(4/8)
36 GCAGGT
BssKI 45 CCAGG ^CC[ACGT]GG
384 CCTGG
BstF5I 51 CATCC GGATG(2/0)
81 CATCC
BstNI 45 CCAGG CC^[AT]GG
384 CCTGG
Cac8I 32 GCTGGC GC[ACGT]^[ACGT]GC
367 GCCCGC
DpnII 103 GATC ^GATC
229 GATC
DraII 128 AGGTCCT [GA]G^G[ACGT]CC[CT]
364 GGGGCCC
EcoO109I 128 AGGTCCT [GA]G^G[ACGT]CC[CT]
364 GGGGCCC
EcoRII 45 CCAGG ^CC[AT]GG
384 CCTGG
EcoT14I 314 CCATGG C^C[AT][AT]GG
398 CCAAGG
Fnu4HI 377 GCCGC GC^[ACGT]GC
380 GCGGC
FokI 51 CATCC GGATG(9/13)
81 CATCC
ItaI 377 GCCGC GC^[ACGT]GC
380 GCGGC
KspI 360 CCGCGG CCGC^GG
378 CCGCGG
MboI 103 GATC ^GATC
229 GATC
MboII 117 GAAGA GAAGA(8/7)
251 TCTTC
MseI 155 TTAA T^TAA
305 TTAA
MspA1I 360 CCGCGG C[AC]G^C[GT]G
378 CCGCGG
MvaI 45 CCAGG CC^[AT]GG
384 CCTGG
MwoI 362 GCGGGGCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
371 GCAAAGGCCGC
NdeII 103 GATC ^GATC
229 GATC
SacII 360 CCGCGG CCGC^GG
378 CCGCGG
Sau3AI 103 GATC ^GATC
229 GATC
ScrFI 45 CCAGG CC^[ACGT]GG
384 CCTGG
StyI 314 CCATGG C^C[AT][AT]GG
398 CCAAGG
Tru9I 155 TTAA T^TAA
305 TTAA
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AccII AciI AfaI AlwI AvaII
BsaJI BsiYI BslI BsrI BstUI
Cfr13I DsaI FauI HaeIII MnlI
MvnI NlaIII NlaIV RsaI Sau96I