Restriction map for
pF1KB6551
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KpnI KspI MaeII MfeI
MluI MluNI MroI MscI MspA1I
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TthHB8I XbaI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 155 CGCG CG^CG
AcsI 522 AAATTC [GA]^AATT[CT]
AhdI 74 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 522 AAATTC [GA]^AATT[CT]
Asp700I 478 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 74 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 899 GGCGCC G^G[CT][GA]CC
BbeI 899 GGCGCC GGCGC^C
BbvCI 505 GCTGAGG CCTCAGC(-5/-2)
BclI 675 TGATCA T^GATCA
BfaI 427 CTAG C^TAG
BglII 841 AGATCT A^GATCT
BsaBI 889 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 62 GAGACG CGTCTC(1/5)
BsrDI 256 GCAATG GCAATG(2/0)
BsrFI 1102 GCCGGC [GA]^CCGG[CT]
BstAPI 552 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 155 CGCG CG^CG
BstXI 624 CCACTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1102 GCCGGC [GA]^CCGG[CT]
Eam1105I 74 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 588 CCTTGG C^C[AT][AT]GG
FbaI 675 TGATCA T^GATCA
HaeII 899 GGCGCC [GA]GCGC^[CT]
HindIII 670 AAGCTT A^AGCTT
HphI 114 TCACC GGTGA(8/7)
KasI 899 GGCGCC G^GCGCC
MaeI 427 CTAG C^TAG
MamI 889 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 385 TTAA T^TAA
MvnI 155 CGCG CG^CG
NaeI 1102 GCCGGC GCC^GGC
NarI 899 GGCGCC GG^CGCC
NgoMIV 1102 GCCGGC G^CCGGC
SfoI 899 GGCGCC GGC^GCC
StyI 588 CCTTGG C^C[AT][AT]GG
Tru9I 385 TTAA T^TAA
TspRI 345 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 478 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 824 GGCGTC G[GA]^CG[CT]C
899 GGCGCC
AfaI 176 GTAC GT^AC
376 GTAC
ApaI 23 GGGCCC GGGCC^C
1033 GGGCCC
AspHI 304 GAGCTC G[AT]GC[AT]^C
1056 GAGCTC
AspI 97 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
935 GACATCGTC
BpmI 978 CTCCAG CTGGAG(16/14)
1044 CTGGAG
BsaHI 824 GGCGTC G[GA]^CG[CT]C
899 GGCGCC
BsaI 233 GAGACC GGTCTC(1/5)
592 GGTCTC
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
1056 GAGCTC
BsrBI 985 GAGCGG CCGCTC(-3/-3)
1014 GAGCGG
BsrI 1043 ACTGG ACTGG(1/-1)
539 CCAGT
BstYI 659 AGATCC [GA]^GATC[CT]
841 AGATCT
EarI 711 GAAGAG CTCTTC(1/4)
1090 GAAGAG
Hin1I 824 GGCGTC G[GA]^CG[CT]C
899 GGCGCC
Ksp632I 711 GAAGAG CTCTTC(1/4)
1090 GAAGAG
MflI 659 AGATCC [GA]^GATC[CT]
841 AGATCT
MslI 598 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
625 CACTTCGGTG
PflFI 97 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
935 GACATCGTC
PflMI 36 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
186 CCACGACTTGG
RsaI 176 GTAC GT^AC
376 GTAC
SacI 304 GAGCTC GAGCT^C
1056 GAGCTC
SmaI 815 CCCGGG CCC^GGG
950 CCCGGG
SmlI 729 CTTGAG C^T[CT][GA]AG
1051 CTTGAG
TfiI 451 GAATC G^A[AT]TC
462 GATTC
Tsp45I 113 GTCAC ^GT[GC]AC
807 GTCAC
Tth111I 97 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
935 GACATCGTC
Van91I 36 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
186 CCACGACTTGG
XcmI 311 CCAAGAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1061 CCAACGGGAAGATGG
XhoII 659 AGATCC [GA]^GATC[CT]
841 AGATCT
XmaCI 815 CCCGGG C^CCGGG
950 CCCGGG
XmaI 815 CCCGGG C^CCGGG
950 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp509I