Restriction map for
pF1KB6514
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I CelII ClaI CpoI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FauI FseI FspI HgaI
Hin1I HindIII KasI KspI MaeI
MamI MfeI MflI MluI MroI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1052 GGTACC G^GTACC
AccI 874 GTCTAC GT^[AC][GT]AC
AcsI 196 GAATTC [GA]^AATT[CT]
AfaI 1053 GTAC GT^AC
AlwI 301 GATCC GGATC(4/5)
AocI 43 CCTCAGG CC^T[ACGT]AGG
ApoI 196 GAATTC [GA]^AATT[CT]
Asp718I 1052 GGTACC G^GTACC
AspHI 387 GAGCTC G[AT]GC[AT]^C
BanI 1052 GGTACC G^G[CT][GA]CC
BanII 387 GAGCTC G[GA]GC[CT]^C
BbvI 915 GCAGC GCAGC(8/12)
BcgI 983 GCAAGGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 510 TGATCA T^GATCA
BmyI 387 GAGCTC G[AGT]GC[ACT]^C
BsaI 369 GGTCTC GGTCTC(1/5)
BsiEI 480 CGGCCG CG[GA][CT]^CG
BsiHKAI 387 GAGCTC G[AT]GC[AT]^C
Bsp1286I 387 GAGCTC G[AGT]GC[ACT]^C
BsrDI 77 GCAATG GCAATG(2/0)
BsrFI 704 ACCGGC [GA]^CCGG[CT]
BstXI 530 CCAGAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 43 CCTCAGG CC^T[ACGT]AGG
Cac8I 1039 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 708 GCGC GCG^C
Cfr10I 704 ACCGGC [GA]^CCGG[CT]
DraII 671 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 480 CGGCCG C^GGCCG
EarI 208 CTCTTC CTCTTC(1/4)
EclXI 480 CGGCCG C^GGCCG
Eco52I 480 CGGCCG C^GGCCG
Eco81I 43 CCTCAGG CC^T[ACGT]AGG
EcoNI 395 CCTCAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 671 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 196 GAATTC G^AATTC
FbaI 510 TGATCA T^GATCA
HaeII 707 GGCGCT [GA]GCGC^[CT]
HapII 705 CCGG C^CGG
HhaI 708 GCGC GCG^C
HinP1I 708 GCGC G^CGC
HpaI 289 GTTAAC GTT^AAC
HpaII 705 CCGG C^CGG
KpnI 1052 GGTACC GGTAC^C
Ksp632I 208 CTCTTC CTCTTC(1/4)
MaeII 947 ACGT A^CGT
MslI 519 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 609 CAGCGG C[AC]G^C[GT]G
MspI 705 CCGG C^CGG
NdeI 374 CATATG CA^TATG
RsaI 1053 GTAC GT^AC
SacI 387 GAGCTC GAGCT^C
TseI 915 GCAGC G^C[AT]GC
TspRI 938 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 15 enzymes) #
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BalI 597 TGGCCA TGG^CCA
1031 TGGCCA
BsmFI 696 GTCCC GGGAC(10/14)
885 GTCCC
DpnII 301 GATC ^GATC
511 GATC
DraI 333 TTTAAA TTT^AAA
1087 TTTAAA
DsaI 35 CCATGG C^C[GA][CT]GG
1028 CCATGG
EcoT14I 35 CCATGG C^C[AT][AT]GG
1028 CCATGG
HincII 163 GTCAAC GT[CT]^[GA]AC
289 GTTAAC
HindII 163 GTCAAC GT[CT]^[GA]AC
289 GTTAAC
MboI 301 GATC ^GATC
511 GATC
MluNI 597 TGGCCA TGG^CCA
1031 TGGCCA
MscI 597 TGGCCA TGG^CCA
1031 TGGCCA
NcoI 35 CCATGG C^CATGG
1028 CCATGG
NdeII 301 GATC ^GATC
511 GATC
Sau3AI 301 GATC ^GATC
511 GATC
StyI 35 CCATGG C^C[AT][AT]GG
1028 CCATGG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwNI AvaII BpmI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
EaeI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
TfiI Tru9I Tsp45I Tsp509I