Restriction map for
pF1KB6510
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
MaeII MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Alw44I 221 GTGCAC G^TGCAC
AlwNI 545 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 221 GTGCAC G^TGCAC
BanI 967 GGTGCC G^G[CT][GA]CC
BfaI 565 CTAG C^TAG
BglI 361 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 262 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 262 GCTAAGC GC^T[ACGT]AGC
BsaBI 679 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 74 ATCGAT AT^CGAT
BspHI 795 TCATGA T^CATGA
BspMI 925 ACCTGC ACCTGC(4/8)
CelII 262 GCTAAGC GC^T[ACGT]AGC
CfoI 7 GCGC GCG^C
ClaI 74 ATCGAT AT^CGAT
DsaI 64 CCGCGG C^C[GA][CT]GG
EarI 667 GAAGAG CTCTTC(1/4)
EcoNI 792 CCTTCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 412 GATATC GAT^ATC
EcoT14I 284 CCAAGG C^C[AT][AT]GG
FauI 63 CCCGC CCCGC(4/6)
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
Ksp632I 667 GAAGAG CTCTTC(1/4)
KspI 64 CCGCGG CCGC^GG
MaeI 565 CTAG C^TAG
MaeIII 697 GTTAC ^GT[ACGT]AC
MamI 679 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 818 TTAA T^TAA
NruI 53 TCGCGA TCG^CGA
PvuII 274 CAGCTG CAG^CTG
RcaI 795 TCATGA T^CATGA
SacI 976 GAGCTC GAGCT^C
SacII 64 CCGCGG CCGC^GG
ScaI 461 AGTACT AGT^ACT
StyI 284 CCAAGG C^C[AT][AT]GG
Tru9I 818 TTAA T^TAA
XcmI 971 CCATTGAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 54 CGCG CG^CG
65 CGCG
AcsI 109 AAATTC [GA]^AATT[CT]
138 GAATTT
AlwI 645 GATCC GGATC(4/5)
249 GGATC
ApoI 109 AAATTC [GA]^AATT[CT]
138 GAATTT
AspHI 221 GTGCAC G[AT]GC[AT]^C
976 GAGCTC
AvaII 48 GGACC G^G[AT]CC
60 GGTCC
BanII 103 GAGCCC G[GA]GC[CT]^C
976 GAGCTC
BcnI 27 CCGGG CC^[GC]GG
750 CCGGG
BglII 171 AGATCT A^GATCT
1017 AGATCT
BpmI 268 CTGGAG CTGGAG(16/14)
730 CTGGAG
BsiHKAI 221 GTGCAC G[AT]GC[AT]^C
976 GAGCTC
BsmAI 69 GAGAC GTCTC(1/5)
197 GTCTC
BstUI 54 CGCG CG^CG
65 CGCG
Cac8I 370 GCCTGC GC[ACGT]^[ACGT]GC
509 GCCTGC
HapII 27 CCGG C^CGG
750 CCGG
HinfI 884 GACTC G^A[ACGT]TC
1023 GACTC
HpaII 27 CCGG C^CGG
750 CCGG
MslI 253 CATGTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
652 CACAGCTATG
MspA1I 64 CCGCGG C[AC]G^C[GT]G
274 CAGCTG
MspI 27 CCGG C^CGG
750 CCGG
MvnI 54 CGCG CG^CG
65 CGCG
NciI 27 CCGGG CC^[GC]GG
750 CCGGG
PleI 884 GACTC GAGTC(4/5)
1023 GACTC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BmyI BsaJI BseRI BsgI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII ItaI MboI MboII MflI
MluNI MnlI MscI MvaI MwoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp509I TspRI TthHB8I
XhoII