Restriction map for
pF1KB6474
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BbvI BcgI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
Fnu4HI FseI FspI HaeII HaeIII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII ItaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspI
StuI StyI SwaI TseI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 616 GTCTAC GT^[AC][GT]AC
Asp700I 867 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 819 GGATCC G^GATCC
BanII 1060 GAGCTC G[GA]GC[CT]^C
BciVI 210 GTATCC GTATCC(6/5)
BclI 546 TGATCA T^GATCA
BsaI 833 GAGACC GGTCTC(1/5)
BsaJI 578 CCTGGG C^C[ACGT][ACGT]GG
Bsp1407I 1007 TGTACA T^GTACA
BsrGI 1007 TGTACA T^GTACA
BssSI 799 CTCGTG CACGAG(-5/-1)
BstBI 914 TTCGAA TT^CGAA
BstXI 852 CCATCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 16 GCGC GCG^C
DraI 430 TTTAAA TTT^AAA
DraII 519 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 46 CTCTTC CTCTTC(1/4)
EcoNI 823 CCTGAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 519 GGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 546 TGATCA T^GATCA
HhaI 16 GCGC GCG^C
HinP1I 16 GCGC G^CGC
Ksp632I 46 CTCTTC CTCTTC(1/4)
MaeIII 949 GTGAC ^GT[ACGT]AC
MwoI 9 GCTGATTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 750 GCATGC [GA]CATG^[CT]
NspV 914 TTCGAA TT^CGAA
PleI 883 GACTC GAGTC(4/5)
PpuMI 519 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1060 GAGCTC GAGCT^C
SfcI 621 CTATAG C^T[GA][CT]AG
SfuI 914 TTCGAA TT^CGAA
SphI 750 GCATGC GCATG^C
SspBI 1007 TGTACA T^GTACA
Tsp45I 949 GTGAC ^GT[GC]AC
XmnI 867 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 316 AAATTT [GA]^AATT[CT]
433 AAATTT
ApoI 316 AAATTT [GA]^AATT[CT]
433 AAATTT
AspHI 526 GAGCAC G[AT]GC[AT]^C
1060 GAGCTC
AvaII 264 GGACC G^G[AT]CC
520 GGACC
BbsI 157 GAAGAC GAAGAC(2/6)
63 GTCTTC
BmyI 526 GAGCAC G[AGT]GC[ACT]^C
1060 GAGCTC
BpuAI 157 GAAGAC GAAGAC(2/6)
63 GTCTTC
BsiHKAI 526 GAGCAC G[AT]GC[AT]^C
1060 GAGCTC
BsmAI 826 GAGAC GTCTC(1/5)
833 GAGAC
Bsp1286I 526 GAGCAC G[AGT]GC[ACT]^C
1060 GAGCTC
BstYI 279 AGATCC [GA]^GATC[CT]
819 GGATCC
Cac8I 720 GCTGGC GC[ACGT]^[ACGT]GC
750 GCATGC
Cfr13I 264 GGACC G^G[ACGT]CC
520 GGACC
MflI 279 AGATCC [GA]^GATC[CT]
819 GGATCC
Sau96I 264 GGACC G^G[ACGT]CC
520 GGACC
XcmI 377 CCATGTACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
410 CCAAGTCCACAGTGG
XhoII 279 AGATCC [GA]^GATC[CT]
819 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI AlwI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
DdeI DpnII EcoRII FokI HinfI
HphI MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfaNI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I