Restriction map for
pF1KB5747
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BglI BglII BlnI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SwaI Tsp45I Tth111I XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1630 GGTACC G^GTACC
AccI 844 GTCTAC GT^[AC][GT]AC
AciI 795 GCGG CCGC(-3/-1)
AlwI 783 GATCC GGATC(4/5)
Aor51HI 419 AGCGCT AGC^GCT
Asp700I 2317 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1630 GGTACC G^GTACC
BalI 577 TGGCCA TGG^CCA
BbsI 2217 GAAGAC GAAGAC(2/6)
BbvCI 1928 CCTCAGC CCTCAGC(-5/-2)
BciVI 472 GTATCC GTATCC(6/5)
BlpI 430 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 430 GCTGAGC GC^T[ACGT]AGC
BpuAI 2217 GAAGAC GAAGAC(2/6)
BsaAI 1526 TACGTA [CT]AC^GT[GA]
BsmI 671 GCATTC GAATGC(1/-1)
BstAPI 671 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 430 GCTGAGC GC^T[ACGT]AGC
CfoI 420 GCGC GCG^C
DraII 309 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 2183 CCATGG C^C[GA][CT]GG
EaeI 577 TGGCCA [CT]^GGCC[GA]
Eco47III 419 AGCGCT AGC^GCT
EcoNI 30 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 309 GGGTCCT [GA]G^G[ACGT]CC[CT]
HaeII 419 AGCGCT [GA]GCGC^[CT]
HhaI 420 GCGC GCG^C
HinP1I 420 GCGC G^CGC
HphI 1903 TCACC GGTGA(8/7)
KpnI 1630 GGTACC GGTAC^C
MaeIII 641 GTTAC ^GT[ACGT]AC
MluNI 577 TGGCCA TGG^CCA
MscI 577 TGGCCA TGG^CCA
NcoI 2183 CCATGG C^CATGG
PpuMI 309 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 619 GAGCTC GAGCT^C
SapI 1093 GAAGAGC GCTCTTC(1/4)
SmlI 616 CTTGAG C^T[CT][GA]AG
SnaBI 1526 TACGTA TAC^GTA
SphI 382 GCATGC GCATG^C
StuI 805 AGGCCT AGG^CCT
TaqI 546 TCGA T^CGA
TthHB8I 546 TCGA T^CGA
XbaI 2103 TCTAGA T^CTAGA
XcmI 2401 CCACCACCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2317 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 2209 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
2315 CAGAATCTG
AspHI 619 GAGCTC G[AT]GC[AT]^C
1562 GTGCTC
BanI 1323 GGTGCC G^G[CT][GA]CC
1630 GGTACC
BanII 619 GAGCTC G[GA]GC[CT]^C
1251 GGGCTC
BcnI 207 CCGGG CC^[GC]GG
786 CCGGG
BseRI 2084 CTCCTC GAGGAG(10/8)
64 GAGGAG
BsiHKAI 619 GAGCTC G[AT]GC[AT]^C
1562 GTGCTC
BsmAI 873 GAGAC GTCTC(1/5)
1647 GTCTC
BstXI 1379 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2404 CCACCAACTTGG
DpnII 783 GATC ^GATC
2065 GATC
EcoRI 1758 GAATTC G^AATTC
1897 GAATTC
HapII 207 CCGG C^CGG
786 CCGG
HpaII 207 CCGG C^CGG
786 CCGG
MaeII 636 ACGT A^CGT
1527 ACGT
MboI 783 GATC ^GATC
2065 GATC
MspI 207 CCGG C^CGG
786 CCGG
NciI 207 CCGGG CC^[GC]GG
786 CCGGG
NdeI 666 CATATG CA^TATG
1505 CATATG
NdeII 783 GATC ^GATC
2065 GATC
NspI 382 GCATGC [GA]CATG^[CT]
1470 GCATGT
PflMI 1181 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1207 CCAATAGTTGG
Sau3AI 783 GATC ^GATC
2065 GATC
SspI 1771 AATATT AAT^ATT
2416 AATATT
Van91I 1181 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1207 CCAATAGTTGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BfaI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeI MboII MnlI MseI MslI
MspA1I MvaI MwoI NlaIII NlaIV
NsiI PleI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI