Restriction map for
pF1KB9783
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BalI BamHI BbeI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspHI BspLU11I BsrDI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I CelII ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HgaI HincII HindII HindIII
HinfI HpaI KasI KpnI MaeI
MamI MboI MfeI MflI MluI
MluNI MscI MseI MslI MunI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI Sau3AI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 541 GACGTC GACGT^C
AccIII 47 TCCGGA T^CCGGA
AcyI 541 GACGTC G[GA]^CG[CT]C
AlwNI 244 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 340 CCTGAGG CC^T[ACGT]AGG
AviII 576 TGCGCA TGC^GCA
BbrPI 120 CACGTG CAC^GTG
BbvCI 718 GCTGAGG CCTCAGC(-5/-2)
BpmI 487 CTGGAG CTGGAG(16/14)
BsaAI 120 CACGTG [CT]AC^GT[GA]
BsaHI 541 GACGTC G[GA]^CG[CT]C
BsaWI 47 TCCGGA [AT]^CCGG[AT]
BseAI 47 TCCGGA T^CCGGA
Bsp1407I 251 TGTACA T^GTACA
BspEI 47 TCCGGA T^CCGGA
BsrBI 412 CCGCTC CCGCTC(-3/-3)
BsrFI 531 ACCGGC [GA]^CCGG[CT]
BsrGI 251 TGTACA T^GTACA
BstEII 657 GGTGACC G^GT[ACGT]ACC
BstPI 657 GGTGACC G^GT[ACGT]ACC
Bsu36I 340 CCTGAGG CC^T[ACGT]AGG
Cfr10I 531 ACCGGC [GA]^CCGG[CT]
EaeI 611 TGGCCG [CT]^GGCC[GA]
Eco81I 340 CCTGAGG CC^T[ACGT]AGG
EcoNI 609 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 657 GGTGACC G^GT[ACGT]ACC
FspI 576 TGCGCA TGC^GCA
Hin1I 541 GACGTC G[GA]^CG[CT]C
KspI 474 CCGCGG CCGC^GG
MroI 47 TCCGGA T^CCGGA
PmaCI 120 CACGTG CAC^GTG
PmlI 120 CACGTG CAC^GTG
PvuII 716 CAGCTG CAG^CTG
SacII 474 CCGCGG CCGC^GG
SmlI 648 CTCAAG C^T[CT][GA]AG
SspBI 251 TGTACA T^GTACA
TspRI 741 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 363 GGCACC G^G[CT][GA]CC
393 GGTGCC
BsaI 188 GAGACC GGTCTC(1/5)
734 GAGACC
BsmAI 188 GAGAC GTCTC(1/5)
734 GAGAC
BstF5I 45 CATCC GGATG(2/0)
100 GGATG
DsaI 35 CCGTGG C^C[GA][CT]GG
474 CCGCGG
EcoT14I 284 CCAAGG C^C[AT][AT]GG
389 CCTTGG
FokI 45 CATCC GGATG(9/13)
100 GGATG
HapII 48 CCGG C^CGG
532 CCGG
HpaII 48 CCGG C^CGG
532 CCGG
MspI 48 CCGG C^CGG
532 CCGG
NlaIII 162 CATG CATG^
258 CATG
SacI 270 GAGCTC GAGCT^C
511 GAGCTC
SapI 133 GAAGAGC GCTCTTC(1/4)
513 GCTCTTC
SbfI 519 CCTGCAGG CCTGCA^GG
546 CCTGCAGG
Sse8387I 519 CCTGCAGG CCTGCA^GG
546 CCTGCAGG
StyI 284 CCAAGG C^C[AT][AT]GG
389 CCTTGG
TaqI 77 TCGA T^CGA
504 TCGA
TthHB8I 77 TCGA T^CGA
504 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AspHI AvaII BanII BbvI BglI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BspMI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EarI EcoO109I EcoRII FauI
Fnu4HI HaeIII HhaI HinP1I HphI
ItaI Ksp632I MaeII MaeIII MboII
MnlI MspA1I MvaI MvnI MwoI
NlaIV PstI RsaI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I