Restriction map for
pF1KB5163
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FauI FokI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI Ksp632I
KspI MamI MluI MluNI MroI
MscI MslI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 37 AAATTC [GA]^AATT[CT]
AflIII 504 ACGTGT A^C[GA][CT]GT
AlwI 174 GGATC GGATC(4/5)
AocI 586 CCTCAGG CC^T[ACGT]AGG
ApoI 37 AAATTC [GA]^AATT[CT]
BbvCI 30 GCTGAGG CCTCAGC(-5/-2)
BcgI 492 GCAGCTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 478 GGATAC GTATCC(6/5)
BclI 86 TGATCA T^GATCA
BcnI 523 CCGGG CC^[GC]GG
BglII 384 AGATCT A^GATCT
BseRI 415 GAGGAG GAGGAG(10/8)
BsmAI 14 GAGAC GTCTC(1/5)
BsmFI 200 GGGAC GGGAC(10/14)
BspMI 208 GCAGGT ACCTGC(4/8)
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BstNI 54 CCTGG CC^[AT]GG
BstYI 384 AGATCT [GA]^GATC[CT]
Bsu36I 586 CCTCAGG CC^T[ACGT]AGG
DsaI 5 CCACGG C^C[GA][CT]GG
Eco81I 586 CCTCAGG CC^T[ACGT]AGG
EcoRII 54 CCTGG ^CC[AT]GG
EcoRV 280 GATATC GAT^ATC
FbaI 86 TGATCA T^GATCA
HapII 523 CCGG C^CGG
HincII 292 GTTAAC GT[CT]^[GA]AC
HindII 292 GTTAAC GT[CT]^[GA]AC
HpaI 292 GTTAAC GTT^AAC
HpaII 523 CCGG C^CGG
HphI 89 TCACC GGTGA(8/7)
MaeII 504 ACGT A^CGT
MaeIII 251 GTGAC ^GT[ACGT]AC
MboII 556 GAAGA GAAGA(8/7)
MflI 384 AGATCT [GA]^GATC[CT]
MseI 293 TTAA T^TAA
MspA1I 28 CCGCTG C[AC]G^C[GT]G
MspI 523 CCGG C^CGG
MvaI 54 CCTGG CC^[AT]GG
NciI 523 CCGGG CC^[GC]GG
PleI 253 GACTC GAGTC(4/5)
SpeI 463 ACTAGT A^CTAGT
Tru9I 293 TTAA T^TAA
Tsp45I 251 GTGAC ^GT[GC]AC
XhoII 384 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 165 GTAC GT^AC
184 GTAC
BsaJI 5 CCACGG C^C[ACGT][ACGT]GG
54 CCTGGG
BsrI 576 ACTGG ACTGG(1/-1)
598 CCAGT
BssKI 54 CCTGG ^CC[ACGT]GG
523 CCGGG
DdeI 31 CTGAG C^T[ACGT]AG
587 CTCAG
MfeI 134 CAATTG C^AATTG
410 CAATTG
MunI 134 CAATTG C^AATTG
410 CAATTG
MwoI 585 GCCTCAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
614 GCTGTTCCTGC
NlaIII 99 CATG CATG^
363 CATG
RsaI 165 GTAC GT^AC
184 GTAC
ScrFI 54 CCTGG CC^[ACGT]GG
523 CCGGG
TaqI 177 TCGA T^CGA
501 TCGA
TspRI 258 CACTG CA[GC]TG[ACGT][ACGT]^
603 CAGTG
TthHB8I 177 TCGA T^CGA
501 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AluI BbvI BfaI DpnII
Fnu4HI HinfI ItaI MaeI MboI
MnlI NdeII Sau3AI TfiI TseI
Tsp509I