Restriction map for
pF1KB5158
##################################
# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BmyI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1286I BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I FauI FbaI
FokI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MamI MluI MroI MslI
MvaI MvnI NaeI NarI NcoI
NgoMIV NheI NlaIV NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 241 AAATTT [GA]^AATT[CT]
AflIII 621 ACACGT A^C[GA][CT]GT
AlwI 264 GATCC GGATC(4/5)
ApoI 241 AAATTT [GA]^AATT[CT]
BalI 188 TGGCCA TGG^CCA
BcnI 459 CCGGG CC^[GC]GG
BsaJI 459 CCGGGG C^C[ACGT][ACGT]GG
BsiYI 191 CCACCGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 191 CCACCGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 146 CGTCTC CGTCTC(1/5)
BsmFI 463 GGGAC GGGAC(10/14)
BsmI 289 GAATGC GAATGC(1/-1)
Bsp1407I 583 TGTACA T^GTACA
BspHI 110 TCATGA T^CATGA
BsrBI 103 GAGCGG CCGCTC(-3/-3)
BsrGI 583 TGTACA T^GTACA
BssKI 459 CCGGG ^CC[ACGT]GG
BstYI 263 AGATCC [GA]^GATC[CT]
Cac8I 46 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 206 GGCCC G^G[ACGT]CC
DpnII 264 GATC ^GATC
EaeI 188 TGGCCA [CT]^GGCC[GA]
EcoT22I 389 ATGCAT ATGCA^T
HapII 459 CCGG C^CGG
HinfI 358 GACTC G^A[ACGT]TC
HpaII 459 CCGG C^CGG
MaeIII 670 GTTAC ^GT[ACGT]AC
MboI 264 GATC ^GATC
MfeI 483 CAATTG C^AATTG
MflI 263 AGATCC [GA]^GATC[CT]
MluNI 188 TGGCCA TGG^CCA
MscI 188 TGGCCA TGG^CCA
MspI 459 CCGG C^CGG
MunI 483 CAATTG C^AATTG
NciI 459 CCGGG CC^[GC]GG
NdeI 368 CATATG CA^TATG
NdeII 264 GATC ^GATC
NsiI 389 ATGCAT ATGCA^T
PleI 358 GACTC GAGTC(4/5)
RcaI 110 TCATGA T^CATGA
Sau3AI 264 GATC ^GATC
Sau96I 206 GGCCC G^G[ACGT]CC
ScrFI 459 CCGGG CC^[ACGT]GG
SfcI 53 CTACAG C^T[GA][CT]AG
SmlI 217 CTTGAG C^T[CT][GA]AG
SspBI 583 TGTACA T^GTACA
TaqI 425 TCGA T^CGA
TspRI 297 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 425 TCGA T^CGA
XhoII 263 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 59 GCGG CCGC(-3/-1)
105 GCGG
BlpI 31 GCTCAGC GC^T[ACGT]AGC
128 GCTCAGC
BpmI 37 CTGGAG CTGGAG(16/14)
181 CTGGAG
Bpu1102I 31 GCTCAGC GC^T[ACGT]AGC
128 GCTCAGC
BseRI 9 GAGGAG GAGGAG(10/8)
40 GAGGAG
BsmAI 356 GAGAC GTCTC(1/5)
147 GTCTC
BsrI 180 ACTGG ACTGG(1/-1)
296 CCAGT
CelII 31 GCTCAGC GC^T[ACGT]AGC
128 GCTCAGC
CfoI 90 GCGC GCG^C
720 GCGC
HhaI 90 GCGC GCG^C
720 GCGC
HinP1I 90 GCGC G^CGC
720 GCGC
MaeII 142 ACGT A^CGT
623 ACGT
MspA1I 34 CAGCTG C[AC]G^C[GT]G
131 CAGCTG
NlaIII 111 CATG CATG^
726 CATG
PvuII 34 CAGCTG CAG^CTG
131 CAGCTG
SfaNI 607 GATGC GCATC(5/9)
201 GCATC
Tsp509I 242 AATT ^AATT
484 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AfaI AluI BbvI DdeI Fnu4HI
HaeIII ItaI MboII MnlI MseI
MwoI RsaI Tru9I TseI