Restriction map for
pF1KB4832
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaII AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiWI
BsmFI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I EarI
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI TfiI TspRI
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 172 GTCGAC GT^[AC][GT]AC
AccII 381 CGCG CG^CG
AcsI 598 AAATTC [GA]^AATT[CT]
ApoI 598 AAATTC [GA]^AATT[CT]
AseI 769 ATTAAT AT^TAAT
AsnI 769 ATTAAT AT^TAAT
Asp700I 364 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 268 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1186 CCCGAG C^[CT]CG[GA]G
BalI 473 TGGCCA TGG^CCA
BamHI 950 GGATCC G^GATCC
BfaI 1069 CTAG C^TAG
BglI 659 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 447 CGGCCG CG[GA][CT]^CG
BsmBI 905 GAGACG CGTCTC(1/5)
BsmI 368 GCATTC GAATGC(1/-1)
BsoBI 1186 CCCGAG C^[CT]CG[GA]G
BsrDI 617 GCAATG GCAATG(2/0)
BsrI 699 ACTGG ACTGG(1/-1)
BstAPI 1167 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 381 CGCG CG^CG
CfoI 380 GCGC GCG^C
DraII 1064 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 447 CGGCCG C^GGCCG
EclXI 447 CGGCCG C^GGCCG
Eco52I 447 CGGCCG C^GGCCG
EcoNI 543 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1064 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 389 CCAAGG C^C[AT][AT]GG
FauI 10 GCGGG CCCGC(4/6)
HgaI 907 GACGC GACGC(5/10)
HhaI 380 GCGC GCG^C
HinP1I 380 GCGC G^CGC
HincII 172 GTCGAC GT[CT]^[GA]AC
HindII 172 GTCGAC GT[CT]^[GA]AC
MaeI 1069 CTAG C^TAG
MluNI 473 TGGCCA TGG^CCA
MscI 473 TGGCCA TGG^CCA
MseI 770 TTAA T^TAA
MslI 930 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 381 CGCG CG^CG
NlaIII 656 CATG CATG^
PflFI 268 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 769 ATTAAT AT^TAAT
PvuII 425 CAGCTG CAG^CTG
SalI 172 GTCGAC G^TCGAC
SspI 772 AATATT AAT^ATT
StuI 110 AGGCCT AGG^CCT
StyI 389 CCAAGG C^C[AT][AT]GG
Tru9I 770 TTAA T^TAA
Tth111I 268 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 689 CCATCTTCCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 364 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 169 GACGTC GACGT^C
628 GACGTC
AcyI 169 GACGTC G[GA]^CG[CT]C
628 GACGTC
AspHI 384 GTGCTC G[AT]GC[AT]^C
1260 GAGCAC
BanI 481 GGCACC G^G[CT][GA]CC
503 GGTGCC
BglII 491 AGATCT A^GATCT
640 AGATCT
BsaHI 169 GACGTC G[GA]^CG[CT]C
628 GACGTC
BseRI 867 CTCCTC GAGGAG(10/8)
532 GAGGAG
BsiHKAI 384 GTGCTC G[AT]GC[AT]^C
1260 GAGCAC
BsrFI 98 ACCGGC [GA]^CCGG[CT]
445 ACCGGC
BstEII 160 GGTTACC G^GT[ACGT]ACC
309 GGTCACC
BstPI 160 GGTTACC G^GT[ACGT]ACC
309 GGTCACC
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
445 ACCGGC
EaeI 447 CGGCCG [CT]^GGCC[GA]
473 TGGCCA
EcoO65I 160 GGTTACC G^GT[ACGT]ACC
309 GGTCACC
Hin1I 169 GACGTC G[GA]^CG[CT]C
628 GACGTC
SexAI 128 ACCTGGT A^CC[AT]GGT
998 ACCTGGT
SfaNI 1155 GATGC GCATC(5/9)
1102 GCATC
TaqI 41 TCGA T^CGA
173 TCGA
Tsp509I 599 AATT ^AATT
768 AATT
TthHB8I 41 TCGA T^CGA
173 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI TseI Tsp45I XhoII