Restriction map for
pF1KB4347
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsiEI BsmAI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeIII MamI MboI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI Sse8387I SspBI SspI StuI
StyI SwaI Tsp45I Tth111I Van91I
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AspHI 131 GTGCTC G[AT]GC[AT]^C
BbvCI 565 CCTCAGC CCTCAGC(-5/-2)
BciVI 180 GGATAC GTATCC(6/5)
BmyI 131 GTGCTC G[AGT]GC[ACT]^C
BsgI 233 GTGCAG GTGCAG(16/14)
BsiHKAI 131 GTGCTC G[AT]GC[AT]^C
BsiWI 5 CGTACG C^GTACG
BsiYI 209 CCATCACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 209 CCATCACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 318 GCATTC GAATGC(1/-1)
Bsp1286I 131 GTGCTC G[AGT]GC[ACT]^C
BsrI 97 CCAGT ACTGG(1/-1)
BssKI 296 CCTGG ^CC[ACGT]GG
BssSI 413 CACGAG CACGAG(-5/-1)
BstAPI 586 GCAACACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 296 CCTGG CC^[AT]GG
Cfr13I 562 GGCCC G^G[ACGT]CC
EcoRII 296 CCTGG ^CC[AT]GG
FauI 311 CCCGC CCCGC(4/6)
HaeIII 562 GGCC GG^CC
HindIII 404 AAGCTT A^AGCTT
HphI 400 GGTGA GGTGA(8/7)
MaeII 442 ACGT A^CGT
MslI 429 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 296 CCTGG CC^[AT]GG
Sau96I 562 GGCCC G^G[ACGT]CC
ScrFI 296 CCTGG CC^[ACGT]GG
SexAI 295 ACCTGGT A^CC[AT]GGT
SfaNI 307 GATGC GCATC(5/9)
SfcI 70 CTACAG C^T[GA][CT]AG
SpeI 249 ACTAGT A^CTAGT
SphI 570 GCATGC GCATG^C
TaqI 135 TCGA T^CGA
TspRI 98 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 135 TCGA T^CGA
XbaI 373 TCTAGA T^CTAGA
XcmI 323 CCAATTCCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 312 CCGC CCGC(-3/-1)
626 GCGG
AcsI 497 AAATTT [GA]^AATT[CT]
509 AAATTC
AfaI 6 GTAC GT^AC
24 GTAC
AluI 243 AGCT AG^CT
405 AGCT
ApoI 497 AAATTT [GA]^AATT[CT]
509 AAATTC
AseI 481 ATTAAT AT^TAAT
532 ATTAAT
AsnI 481 ATTAAT AT^TAAT
532 ATTAAT
Asp700I 196 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
505 GAAAAAATTC
BfaI 250 CTAG C^TAG
374 CTAG
DdeI 449 CTAAG C^T[ACGT]AG
566 CTCAG
MaeI 250 CTAG C^TAG
374 CTAG
NspI 570 GCATGC [GA]CATG^[CT]
591 ACATGC
PleI 425 GACTC GAGTC(4/5)
521 GAGTC
PshBI 481 ATTAAT AT^TAAT
532 ATTAAT
RsaI 6 GTAC GT^AC
24 GTAC
TfiI 196 GAATC G^A[AT]TC
370 GAATC
XmnI 196 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
505 GAAAAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BbvI Cac8I EarI Fnu4HI HinfI
ItaI Ksp632I MboII MnlI MseI
MwoI NlaIII Tru9I TseI Tsp509I