Restriction map for
pF1KB9943
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI MaeII MluI
MroI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 661 GTAGAC GT^[AC][GT]AC
AcyI 99 GACGCC G[GA]^CG[CT]C
AlwI 1354 GATCC GGATC(4/5)
AlwNI 1418 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 383 TGGCCA TGG^CCA
BanI 742 GGTGCC G^G[CT][GA]CC
BbsI 552 GAAGAC GAAGAC(2/6)
BclI 759 TGATCA T^GATCA
BpmI 1464 CTGGAG CTGGAG(16/14)
BpuAI 552 GAAGAC GAAGAC(2/6)
BsaBI 754 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 99 GACGCC G[GA]^CG[CT]C
BsmAI 1467 GAGAC GTCTC(1/5)
BsmFI 82 GGGAC GGGAC(10/14)
BsrFI 85 ACCGGC [GA]^CCGG[CT]
BstAPI 306 GCATAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 750 GGATG GGATG(2/0)
BstYI 1353 AGATCC [GA]^GATC[CT]
Cfr10I 85 ACCGGC [GA]^CCGG[CT]
DsaI 41 CCGCGG C^C[GA][CT]GG
EcoRI 146 GAATTC G^AATTC
FauI 67 GCGGG CCCGC(4/6)
FbaI 759 TGATCA T^GATCA
FokI 750 GGATG GGATG(9/13)
HgaI 99 GACGC GACGC(5/10)
Hin1I 99 GACGCC G[GA]^CG[CT]C
KspI 41 CCGCGG CCGC^GG
MamI 754 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 379 CAATTG C^AATTG
MflI 1353 AGATCC [GA]^GATC[CT]
MluNI 383 TGGCCA TGG^CCA
MscI 383 TGGCCA TGG^CCA
MspA1I 41 CCGCGG C[AC]G^C[GT]G
MunI 379 CAATTG C^AATTG
PflMI 1379 CCAAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 916 GAGTC GAGTC(4/5)
SacII 41 CCGCGG CCGC^GG
ScaI 133 AGTACT AGT^ACT
SfaNI 417 GCATC GCATC(5/9)
SfcI 659 CTGTAG C^T[GA][CT]AG
SmlI 645 CTCAAG C^T[CT][GA]AG
TfiI 339 GATTC G^A[AT]TC
Tsp45I 290 GTGAC ^GT[GC]AC
TspRI 8 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 1379 CCAAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1353 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 42 CGCG CG^CG
72 CGCG
AvaII 83 GGACC G^G[AT]CC
1411 GGACC
BcnI 50 CCGGG CC^[GC]GG
80 CCGGG
BstUI 42 CGCG CG^CG
72 CGCG
DdeI 194 CTAAG C^T[ACGT]AG
463 CTCAG
DpnII 760 GATC ^GATC
1354 GATC
DraI 229 TTTAAA TTT^AAA
505 TTTAAA
HaeII 105 AGCGCC [GA]GCGC^[CT]
1175 AGCGCC
HindIII 629 AAGCTT A^AGCTT
1076 AAGCTT
HinfI 339 GATTC G^A[ACGT]TC
916 GAGTC
HphI 780 GGTGA GGTGA(8/7)
762 TCACC
MaeIII 290 GTGAC ^GT[ACGT]AC
670 GTAAC
MboI 760 GATC ^GATC
1354 GATC
MslI 394 CATAATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
993 CACACCTGTG
MvnI 42 CGCG CG^CG
72 CGCG
NciI 50 CCGGG CC^[GC]GG
80 CCGGG
NdeII 760 GATC ^GATC
1354 GATC
NlaIV 82 GGGACC GG[ACGT]^[ACGT]CC
742 GGTGCC
SapI 511 GAAGAGC GCTCTTC(1/4)
532 GAAGAGC
Sau3AI 760 GATC ^GATC
1354 GATC
XbaI 825 TCTAGA T^CTAGA
897 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BbvI BfaI BsaJI BsiYI
BslI BsrI BssKI BstNI Cac8I
CfoI Cfr13I EaeI EarI EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI Ksp632I MaeI MboII
MnlI MseI MspI MvaI MwoI
NlaIII RsaI Sau96I ScrFI Tru9I
TseI Tsp509I XmnI