Restriction map for
pF1KB0465
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiEI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI NarI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 190 GACGTC GACGT^C
Acc65I 254 GGTACC G^GTACC
AccI 919 GTCTAC GT^[AC][GT]AC
AccII 1330 CGCG CG^CG
AcyI 190 GACGTC G[GA]^CG[CT]C
AflIII 407 ACGTGT A^C[GA][CT]GT
Alw44I 988 GTGCAC G^TGCAC
AlwI 1302 GATCC GGATC(4/5)
ApaLI 988 GTGCAC G^TGCAC
Asp718I 254 GGTACC G^GTACC
AspHI 988 GTGCAC G[AT]GC[AT]^C
BanI 254 GGTACC G^G[CT][GA]CC
BbvCI 102 GCTGAGG CCTCAGC(-5/-2)
BciVI 181 GTATCC GTATCC(6/5)
BclI 863 TGATCA T^GATCA
BcnI 351 CCCGG CC^[GC]GG
BfaI 1375 CTAG C^TAG
BlpI 1133 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1133 GCTCAGC GC^T[ACGT]AGC
BsaAI 406 TACGTG [CT]AC^GT[GA]
BsaHI 190 GACGTC G[GA]^CG[CT]C
BsgI 535 CTGCAC GTGCAG(16/14)
BsiHKAI 988 GTGCAC G[AT]GC[AT]^C
BsiWI 404 CGTACG C^GTACG
BspHI 608 TCATGA T^CATGA
BsrBI 95 CCGCTC CCGCTC(-3/-3)
BstUI 1330 CGCG CG^CG
BstXI 44 CCACACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1301 AGATCC [GA]^GATC[CT]
CelII 1133 GCTCAGC GC^T[ACGT]AGC
EcoNI 51 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1139 CCATGG C^C[AT][AT]GG
FbaI 863 TGATCA T^GATCA
HgaI 1029 GCGTC GACGC(5/10)
Hin1I 190 GACGTC G[GA]^CG[CT]C
HincII 1080 GTCAAC GT[CT]^[GA]AC
HindII 1080 GTCAAC GT[CT]^[GA]AC
KpnI 254 GGTACC GGTAC^C
MaeI 1375 CTAG C^TAG
MflI 1301 AGATCC [GA]^GATC[CT]
MslI 768 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1330 CGCG CG^CG
NciI 351 CCCGG CC^[GC]GG
NcoI 1139 CCATGG C^CATGG
NheI 1374 GCTAGC G^CTAGC
RcaI 608 TCATGA T^CATGA
SapI 1185 GAAGAGC GCTCTTC(1/4)
ScaI 263 AGTACT AGT^ACT
SexAI 1034 ACCTGGT A^CC[AT]GGT
SgrAI 715 CGCCGGCG C[GA]^CCGG[CT]G
StuI 1229 AGGCCT AGG^CCT
StyI 1139 CCATGG C^C[AT][AT]GG
Tsp509I 1280 AATT ^AATT
XcmI 206 CCATCCTCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1301 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AluI 1262 AGCT AG^CT
1352 AGCT
AocI 295 CCTGAGG CC^T[ACGT]AGG
1068 CCTGAGG
BanII 1188 GAGCCC G[GA]GC[CT]^C
1335 GGGCTC
BbsI 604 GTCTTC GAAGAC(2/6)
856 GTCTTC
BglI 1017 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1231 GCCTCGCTGGC
BpuAI 604 GTCTTC GAAGAC(2/6)
856 GTCTTC
BsaI 964 GAGACC GGTCTC(1/5)
819 GGTCTC
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
1068 CCTGAGG
DpnII 864 GATC ^GATC
1302 GATC
DrdI 190 GACGTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
595 GACAGGAGTGTC
DsaI 332 CCACGG C^C[GA][CT]GG
1139 CCATGG
EaeI 887 CGGCCA [CT]^GGCC[GA]
959 TGGCCG
EarI 727 CTCTTC CTCTTC(1/4)
1185 GAAGAG
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
1068 CCTGAGG
FauI 94 CCCGC CCCGC(4/6)
1293 GCGGG
HaeII 21 GGCGCT [GA]GCGC^[CT]
569 GGCGCT
HinfI 228 GAATC G^A[ACGT]TC
1049 GAATC
Ksp632I 727 CTCTTC CTCTTC(1/4)
1185 GAAGAG
MboI 864 GATC ^GATC
1302 GATC
NaeI 716 GCCGGC GCC^GGC
885 GCCGGC
NdeII 864 GATC ^GATC
1302 GATC
NgoMIV 716 GCCGGC G^CCGGC
885 GCCGGC
PpuMI 347 GGGTCCC [GA]G^G[AT]CC[CT]
1167 AGGACCC
Sau3AI 864 GATC ^GATC
1302 GATC
TfiI 228 GAATC G^A[AT]TC
1049 GAATC
Tsp45I 460 GTGAC ^GT[GC]AC
1031 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AlwNI AvaI AvaII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboII MnlI MspI
MvaI MwoI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfaNI SfcI
TaqI TseI TspRI TthHB8I