Restriction map for
pF1KB6206
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BseAI BsiEI
BsmBI BsmFI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspI PacI PaeR7I
PinAI PmeI PshAI PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 2121 GCGG CCGC(-3/-1)
AclI 697 AACGTT AA^CGTT
Alw44I 1395 GTGCAC G^TGCAC
AlwI 1259 GATCC GGATC(4/5)
ApaLI 1395 GTGCAC G^TGCAC
AseI 601 ATTAAT AT^TAAT
AsnI 601 ATTAAT AT^TAAT
AspI 1180 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 239 CCCGAG C^[CT]CG[GA]G
BbrPI 316 CACGTG CAC^GTG
BsaAI 316 CACGTG [CT]AC^GT[GA]
BsaWI 339 ACCGGA [AT]^CCGG[AT]
BsiWI 1724 CGTACG C^GTACG
BsoBI 239 CCCGAG C^[CT]CG[GA]G
BspHI 606 TCATGA T^CATGA
BstAPI 1901 GCAATCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1138 TTCGAA TT^CGAA
BstF5I 51 GGATG GGATG(2/0)
BstYI 1258 AGATCC [GA]^GATC[CT]
DsaI 1007 CCATGG C^C[GA][CT]GG
EcoNI 1919 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1007 CCATGG C^C[AT][AT]GG
EcoT22I 1945 ATGCAT ATGCA^T
FokI 51 GGATG GGATG(9/13)
MflI 1258 AGATCC [GA]^GATC[CT]
NcoI 1007 CCATGG C^CATGG
NsiI 1945 ATGCAT ATGCA^T
NspV 1138 TTCGAA TT^CGAA
PflFI 1180 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 312 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 316 CACGTG CAC^GTG
PmlI 316 CACGTG CAC^GTG
PpuMI 209 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 601 ATTAAT AT^TAAT
Psp1406I 697 AACGTT AA^CGTT
RcaI 606 TCATGA T^CATGA
SfcI 1046 CTACAG C^T[GA][CT]AG
SfuI 1138 TTCGAA TT^CGAA
StyI 1007 CCATGG C^C[AT][AT]GG
Tth111I 1180 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 312 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1258 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanII 1929 GAGCTC G[GA]GC[CT]^C
2013 GAGCTC
BbvI 2094 GCAGC GCAGC(8/12)
997 GCTGC
BfaI 296 CTAG C^TAG
656 CTAG
BsaI 133 GAGACC GGTCTC(1/5)
967 GAGACC
BsgI 216 GTGCAG GTGCAG(16/14)
906 GTGCAG
BsmI 1318 GAATGC GAATGC(1/-1)
64 GCATTC
DraII 209 AGGACCT [GA]G^G[ACGT]CC[CT]
233 AGGCCCC
DraIII 313 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
841 CACAGTGTG
EarI 832 CTCTTC CTCTTC(1/4)
205 GAAGAG
EcoO109I 209 AGGACCT [GA]G^G[ACGT]CC[CT]
233 AGGCCCC
Fnu4HI 997 GCTGC GC^[ACGT]GC
2094 GCAGC
HapII 340 CCGG C^CGG
2091 CCGG
HpaII 340 CCGG C^CGG
2091 CCGG
ItaI 997 GCTGC GC^[ACGT]GC
2094 GCAGC
Ksp632I 832 CTCTTC CTCTTC(1/4)
205 GAAGAG
MaeI 296 CTAG C^TAG
656 CTAG
MspI 340 CCGG C^CGG
2091 CCGG
MwoI 1854 GCTCATCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1901 GCAATCAGTGC
SacI 1929 GAGCTC GAGCT^C
2013 GAGCTC
TseI 997 GCTGC G^C[AT]GC
2094 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AspHI AvaII BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstNI Cfr13I DdeI DpnII EcoRI
EcoRII HaeIII HinfI HphI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SspBI
TaqI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XmnI