Restriction map for
pF1KB9886
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BbeI BbrPI BciVI
BclI BfrI BlnI BsaBI BseAI
BsiEI BsiWI BspEI BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI FbaI FseI HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NdeI NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tth111I XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 187 GTCTAC GT^[AC][GT]AC
AcsI 1417 GAATTT [GA]^AATT[CT]
AflIII 746 ACATGT A^C[GA][CT]GT
AocI 967 CCTGAGG CC^T[ACGT]AGG
ApaI 845 GGGCCC GGGCC^C
ApoI 1417 GAATTT [GA]^AATT[CT]
AviII 1044 TGCGCA TGC^GCA
BanII 845 GGGCCC G[GA]GC[CT]^C
BbvCI 655 CCTCAGC CCTCAGC(-5/-2)
BcgI 29 CGAGCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 258 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 258 GCTCAGC GC^T[ACGT]AGC
BsaAI 79 TACGTG [CT]AC^GT[GA]
BsaI 389 GAGACC GGTCTC(1/5)
BsaWI 451 TCCGGT [AT]^CCGG[AT]
BsgI 1915 CTGCAC GTGCAG(16/14)
Bsp1407I 551 TGTACA T^GTACA
BspDI 1426 ATCGAT AT^CGAT
BspHI 620 TCATGA T^CATGA
BspLU11I 746 ACATGT A^CATGT
BsrGI 551 TGTACA T^GTACA
BstAPI 600 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1186 TTCGAA TT^CGAA
Bsu36I 967 CCTGAGG CC^T[ACGT]AGG
CelII 258 GCTCAGC GC^T[ACGT]AGC
ClaI 1426 ATCGAT AT^CGAT
DrdI 1027 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 707 TGGCCG [CT]^GGCC[GA]
Eco81I 967 CCTGAGG CC^T[ACGT]AGG
EcoRV 1600 GATATC GAT^ATC
FspI 1044 TGCGCA TGC^GCA
HaeII 147 GGCGCT [GA]GCGC^[CT]
HincII 1069 GTTGAC GT[CT]^[GA]AC
HindII 1069 GTTGAC GT[CT]^[GA]AC
HindIII 2012 AAGCTT A^AGCTT
NaeI 409 GCCGGC GCC^GGC
NgoMIV 409 GCCGGC G^CCGGC
NspV 1186 TTCGAA TT^CGAA
RcaI 620 TCATGA T^CATGA
ScaI 542 AGTACT AGT^ACT
SfuI 1186 TTCGAA TT^CGAA
SmaI 427 CCCGGG CCC^GGG
SspBI 551 TGTACA T^GTACA
StuI 1277 AGGCCT AGG^CCT
TfiI 2148 GAATC G^A[AT]TC
Tsp45I 910 GTGAC ^GT[GC]AC
XbaI 2131 TCTAGA T^CTAGA
XmaCI 427 CCCGGG C^CCGGG
XmaI 427 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 531 GACGCC G[GA]^CG[CT]C
1743 GACGCC
Alw44I 607 GTGCAC G^TGCAC
1269 GTGCAC
ApaLI 607 GTGCAC G^TGCAC
1269 GTGCAC
BamHI 1007 GGATCC G^GATCC
1998 GGATCC
BanI 433 GGTGCC G^G[CT][GA]CC
1348 GGTGCC
BglI 404 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
654 GCCTCAGCGGC
BglII 227 AGATCT A^GATCT
2115 AGATCT
BsaHI 531 GACGCC G[GA]^CG[CT]C
1743 GACGCC
BsmI 2028 GAATGC GAATGC(1/-1)
2124 GAATGC
BsrDI 1041 CATTGC GCAATG(2/0)
1582 CATTGC
EcoT22I 2030 ATGCAT ATGCA^T
2126 ATGCAT
Hin1I 531 GACGCC G[GA]^CG[CT]C
1743 GACGCC
NsiI 2030 ATGCAT ATGCA^T
2126 ATGCAT
PaeR7I 893 CTCGAG C^TCGAG
1402 CTCGAG
PleI 52 GAGTC GAGTC(4/5)
368 GAGTC
PpuMI 116 GGGACCT [GA]G^G[AT]CC[CT]
560 GGGACCT
PstI 513 CTGCAG CTGCA^G
598 CTGCAG
XcmI 1862 CCAAACAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1871 CCATGGGCTTCTTGG
XhoI 893 CTCGAG C^TCGAG
1402 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp509I TspRI TthHB8I Van91I XhoII