Restriction map for
pF1KB4124
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BclI BcnI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRV FauI
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 17 GGTACC G^GTACC
AccI 1488 GTCTAC GT^[AC][GT]AC
AflII 1269 CTTAAG C^TTAAG
AocI 315 CCTGAGG CC^T[ACGT]AGG
AseI 1504 ATTAAT AT^TAAT
AsnI 1504 ATTAAT AT^TAAT
Asp700I 1132 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 17 GGTACC G^GTACC
AvaI 2324 CCCGAG C^[CT]CG[GA]G
BcgI 1321 GCAGCTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1159 GGATAC GTATCC(6/5)
BfrI 1269 CTTAAG C^TTAAG
BglI 2567 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 867 AGATCT A^GATCT
BsaWI 2414 ACCGGA [AT]^CCGG[AT]
BseRI 918 CTCCTC GAGGAG(10/8)
BsoBI 2324 CCCGAG C^[CT]CG[GA]G
BspMI 1211 ACCTGC ACCTGC(4/8)
BstBI 2666 TTCGAA TT^CGAA
BstEII 472 GGTGACC G^GT[ACGT]ACC
BstPI 472 GGTGACC G^GT[ACGT]ACC
BstYI 867 AGATCT [GA]^GATC[CT]
Bsu36I 315 CCTGAGG CC^T[ACGT]AGG
CfoI 79 GCGC GCG^C
DraI 1783 TTTAAA TTT^AAA
Eco81I 315 CCTGAGG CC^T[ACGT]AGG
EcoO65I 472 GGTGACC G^GT[ACGT]ACC
EcoT22I 2144 ATGCAT ATGCA^T
HapII 2415 CCGG C^CGG
HhaI 79 GCGC GCG^C
HinP1I 79 GCGC G^CGC
HincII 2281 GTCAAC GT[CT]^[GA]AC
HindII 2281 GTCAAC GT[CT]^[GA]AC
HindIII 496 AAGCTT A^AGCTT
HpaII 2415 CCGG C^CGG
KpnI 17 GGTACC GGTAC^C
MaeII 974 ACGT A^CGT
MflI 867 AGATCT [GA]^GATC[CT]
MspI 2415 CCGG C^CGG
NcoI 2558 CCATGG C^CATGG
NheI 1215 GCTAGC G^CTAGC
NsiI 2144 ATGCAT ATGCA^T
NspV 2666 TTCGAA TT^CGAA
PshBI 1504 ATTAAT AT^TAAT
SapI 1765 GAAGAGC GCTCTTC(1/4)
SexAI 212 ACCAGGT A^CC[AT]GGT
SfuI 2666 TTCGAA TT^CGAA
SphI 979 GCATGC GCATG^C
StuI 988 AGGCCT AGG^CCT
XbaI 2652 TCTAGA T^CTAGA
XhoII 867 AGATCT [GA]^GATC[CT]
XmnI 1132 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 697 ACATGT A^C[GA][CT]GT
2501 ACATGT
AlwNI 653 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
2345 CAGAAGCTG
AspHI 876 GTGCTC G[AT]GC[AT]^C
2939 GAGCAC
BanI 17 GGTACC G^G[CT][GA]CC
1795 GGCACC
BbsI 332 GTCTTC GAAGAC(2/6)
855 GTCTTC
BpuAI 332 GTCTTC GAAGAC(2/6)
855 GTCTTC
BsiHKAI 876 GTGCTC G[AT]GC[AT]^C
2939 GAGCAC
BspHI 2105 TCATGA T^CATGA
2915 TCATGA
BspLU11I 697 ACATGT A^CATGT
2501 ACATGT
DraII 1829 AGGACCC [GA]G^G[ACGT]CC[CT]
2255 GGGACCT
DsaI 840 CCGTGG C^C[GA][CT]GG
2558 CCATGG
EaeI 837 TGGCCG [CT]^GGCC[GA]
1932 TGGCCG
EarI 2723 CTCTTC CTCTTC(1/4)
1765 GAAGAG
EcoO109I 1829 AGGACCC [GA]G^G[ACGT]CC[CT]
2255 GGGACCT
EcoRI 1912 GAATTC G^AATTC
2648 GAATTC
Ksp632I 2723 CTCTTC CTCTTC(1/4)
1765 GAAGAG
PleI 1053 GAGTC GAGTC(4/5)
1412 GAGTC
PpuMI 1829 AGGACCC [GA]G^G[AT]CC[CT]
2255 GGGACCT
PshAI 524 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
696 GACATGTGTC
RcaI 2105 TCATGA T^CATGA
2915 TCATGA
SmlI 863 CTCAAG C^T[CT][GA]AG
1269 CTTAAG
SspI 1098 AATATT AAT^ATT
2272 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI