Restriction map for
pF1KB4094
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI BamHI BcgI BciVI BclI
BfrI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI HindIII HpaI KspI
MamI MfeI MluI MroI MseI
MunI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SacII SapI ScaI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tth111I XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 839 GGTACC G^GTACC
AccI 301 GTCGAC GT^[AC][GT]AC
AccII 154 CGCG CG^CG
AclI 169 AACGTT AA^CGTT
AcyI 295 GGCGCC G[GA]^CG[CT]C
AflIII 977 ACATGT A^C[GA][CT]GT
AocI 851 CCTTAGG CC^T[ACGT]AGG
Asp718I 839 GGTACC G^GTACC
AviII 1379 TGCGCA TGC^GCA
AvrII 1344 CCTAGG C^CTAGG
BalI 1712 TGGCCA TGG^CCA
BbeI 295 GGCGCC GGCGC^C
BbrPI 1312 CACGTG CAC^GTG
BlnI 1344 CCTAGG C^CTAGG
BlpI 661 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 661 GCTGAGC GC^T[ACGT]AGC
BsaAI 1312 CACGTG [CT]AC^GT[GA]
BsaHI 295 GGCGCC G[GA]^CG[CT]C
BsaWI 478 TCCGGT [AT]^CCGG[AT]
BspLU11I 977 ACATGT A^CATGT
BstEII 600 GGTCACC G^GT[ACGT]ACC
BstPI 600 GGTCACC G^GT[ACGT]ACC
BstUI 154 CGCG CG^CG
BstXI 1154 CCAGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 851 CCTTAGG CC^T[ACGT]AGG
CelII 661 GCTGAGC GC^T[ACGT]AGC
EarI 427 CTCTTC CTCTTC(1/4)
Eco81I 851 CCTTAGG CC^T[ACGT]AGG
EcoO65I 600 GGTCACC G^GT[ACGT]ACC
FseI 895 GGCCGGCC GGCCGG^CC
FspI 1379 TGCGCA TGC^GCA
Hin1I 295 GGCGCC G[GA]^CG[CT]C
KasI 295 GGCGCC G^GCGCC
KpnI 839 GGTACC GGTAC^C
Ksp632I 427 CTCTTC CTCTTC(1/4)
MluNI 1712 TGGCCA TGG^CCA
MscI 1712 TGGCCA TGG^CCA
MslI 1299 CACGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 154 CGCG CG^CG
NarI 295 GGCGCC GG^CGCC
NcoI 845 CCATGG C^CATGG
NdeI 372 CATATG CA^TATG
PflMI 510 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1312 CACGTG CAC^GTG
PmlI 1312 CACGTG CAC^GTG
Psp1406I 169 AACGTT AA^CGTT
SacI 178 GAGCTC GAGCT^C
SalI 301 GTCGAC G^TCGAC
SbfI 1026 CCTGCAGG CCTGCA^GG
SexAI 98 ACCTGGT A^CC[AT]GGT
SfoI 295 GGCGCC GGC^GCC
SmaI 1439 CCCGGG CCC^GGG
Sse8387I 1026 CCTGCAGG CCTGCA^GG
TfiI 1259 GATTC G^A[AT]TC
Tsp509I 467 AATT ^AATT
Van91I 510 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 870 TCTAGA T^CTAGA
XmaCI 1439 CCCGGG C^CCGGG
XmaI 1439 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 511 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
796 CAGAAGCTG
BbsI 21 GAAGAC GAAGAC(2/6)
1081 GAAGAC
BglII 867 AGATCT A^GATCT
1091 AGATCT
BpuAI 21 GAAGAC GAAGAC(2/6)
1081 GAAGAC
BsaI 360 GGTCTC GGTCTC(1/5)
520 GGTCTC
BsiEI 1180 CGGCCG CG[GA][CT]^CG
1406 CGGCCG
BsrDI 930 CATTGC GCAATG(2/0)
1010 GCAATG
BstAPI 795 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1371 GCAGAAGGTGC
EagI 1180 CGGCCG C^GGCCG
1406 CGGCCG
EclXI 1180 CGGCCG C^GGCCG
1406 CGGCCG
Eco52I 1180 CGGCCG C^GGCCG
1406 CGGCCG
HaeII 295 GGCGCC [GA]GCGC^[CT]
1732 AGCGCC
HgaI 24 GACGC GACGC(5/10)
155 GCGTC
HincII 148 GTCAAC GT[CT]^[GA]AC
301 GTCGAC
HindII 148 GTCAAC GT[CT]^[GA]AC
301 GTCGAC
NaeI 896 GCCGGC GCC^GGC
1448 GCCGGC
NgoMIV 896 GCCGGC G^CCGGC
1448 GCCGGC
NspI 695 ACATGC [GA]CATG^[CT]
977 ACATGT
PaeR7I 320 CTCGAG C^TCGAG
1728 CTCGAG
PleI 769 GACTC GAGTC(4/5)
1471 GAGTC
PpuMI 1229 AGGACCC [GA]G^G[AT]CC[CT]
1551 AGGACCC
PvuII 593 CAGCTG CAG^CTG
787 CAGCTG
SfaNI 1271 GCATC GCATC(5/9)
1639 GCATC
SfiI 504 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1398 GGCCGAGGCGGCC
StuI 1031 AGGCCT AGG^CCT
1839 AGGCCT
XcmI 44 CCATCGACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
338 CCAGCCGTGAGCTGG
XhoI 320 CTCGAG C^TCGAG
1728 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII