Restriction map for
pF1KB7591
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI Ksp632I
KspI MaeI MaeII MamI MboII
MfeI MflI MluI MluNI MroI
MscI MseI MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 581 GGTACC G^GTACC
Aor51HI 383 AGCGCT AGC^GCT
ApaI 290 GGGCCC GGGCC^C
Asp718I 581 GGTACC G^GTACC
AspHI 116 GTGCTC G[AT]GC[AT]^C
AvaII 89 GGACC G^G[AT]CC
BanII 290 GGGCCC G[GA]GC[CT]^C
BbvCI 648 CCTCAGC CCTCAGC(-5/-2)
BlpI 364 GCTAAGC GC^T[ACGT]AGC
BpmI 250 CTGGAG CTGGAG(16/14)
Bpu1102I 364 GCTAAGC GC^T[ACGT]AGC
BsiHKAI 116 GTGCTC G[AT]GC[AT]^C
BspMI 493 ACCTGC ACCTGC(4/8)
BsrFI 607 GCCGGC [GA]^CCGG[CT]
BstF5I 662 CATCC GGATG(2/0)
BstXI 98 CCACCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 364 GCTAAGC GC^T[ACGT]AGC
Cfr10I 607 GCCGGC [GA]^CCGG[CT]
DpnII 158 GATC ^GATC
DraII 88 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 101 CCACGG C^C[GA][CT]GG
Eco47III 383 AGCGCT AGC^GCT
EcoO109I 88 GGGACCC [GA]G^G[ACGT]CC[CT]
FokI 662 CATCC GGATG(9/13)
HphI 504 TCACC GGTGA(8/7)
KpnI 581 GGTACC GGTAC^C
MboI 158 GATC ^GATC
MslI 99 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 247 CAGCTG C[AC]G^C[GT]G
NaeI 607 GCCGGC GCC^GGC
NdeII 158 GATC ^GATC
NgoMIV 607 GCCGGC G^CCGGC
PaeR7I 111 CTCGAG C^TCGAG
PflMI 665 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 88 GGGACCC [GA]G^G[AT]CC[CT]
PstI 126 CTGCAG CTGCA^G
PvuII 247 CAGCTG CAG^CTG
Sau3AI 158 GATC ^GATC
SmaI 308 CCCGGG CCC^GGG
TaqI 112 TCGA T^CGA
TfiI 335 GAATC G^A[AT]TC
TthHB8I 112 TCGA T^CGA
Van91I 665 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 661 CCATCCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 111 CTCGAG C^TCGAG
XmaCI 308 CCCGGG C^CCGGG
XmaI 308 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 276 GTAC GT^AC
582 GTAC
AlwNI 397 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
599 CAGCCCCTG
BmyI 116 GTGCTC G[AGT]GC[ACT]^C
290 GGGCCC
BsgI 143 GTGCAG GTGCAG(16/14)
235 GTGCAG
BsmAI 72 GAGAC GTCTC(1/5)
414 GTCTC
BsmFI 88 GGGAC GGGAC(10/14)
311 GGGAC
Bsp1286I 116 GTGCTC G[AGT]GC[ACT]^C
290 GGGCCC
EaeI 80 CGGCCA [CT]^GGCC[GA]
609 CGGCCA
FauI 171 CCCGC CCCGC(4/6)
195 CCCGC
MaeIII 490 GTGAC ^GT[ACGT]AC
561 GTCAC
NlaIII 517 CATG CATG^
639 CATG
RsaI 276 GTAC GT^AC
582 GTAC
SfcI 126 CTGCAG C^T[GA][CT]AG
510 CTACAG
SmlI 111 CTCGAG C^T[CT][GA]AG
655 CTTGAG
Tsp45I 490 GTGAC ^GT[GC]AC
561 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcyI AluI AvaI BanI
BbeI BbvI BcnI BsaHI BsaJI
BsiYI BslI BsoBI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
EcoNI EcoRII Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MnlI MspI
MvaI MwoI NarI NciI NlaIV
PleI Sau96I ScrFI SfoI TseI
TspRI