Restriction map for
pF1KA1339
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BalI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 929 GACGTC GACGT^C
AccI 906 GTCTAC GT^[AC][GT]AC
AcyI 929 GACGTC G[GA]^CG[CT]C
AlwNI 935 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1301 AGCGCT AGC^GCT
ApaI 725 GGGCCC GGGCC^C
BamHI 377 GGATCC G^GATCC
BanI 420 GGTGCC G^G[CT][GA]CC
BglI 145 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 929 GACGTC G[GA]^CG[CT]C
BsaI 1882 GAGACC GGTCTC(1/5)
Bsp1407I 755 TGTACA T^GTACA
BspHI 512 TCATGA T^CATGA
BspLU11I 1064 ACATGT A^CATGT
BsrGI 755 TGTACA T^GTACA
DraIII 1202 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 542 CCATGG C^C[GA][CT]GG
Eco47III 1301 AGCGCT AGC^GCT
EcoNI 303 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 409 GATATC GAT^ATC
FauI 1422 GCGGG CCCGC(4/6)
Hin1I 929 GACGTC G[GA]^CG[CT]C
HincII 1864 GTCAAC GT[CT]^[GA]AC
HindII 1864 GTCAAC GT[CT]^[GA]AC
NaeI 182 GCCGGC GCC^GGC
NcoI 542 CCATGG C^CATGG
NgoMIV 182 GCCGGC G^CCGGC
NspI 1064 ACATGT [GA]CATG^[CT]
PflMI 1886 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1824 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 512 TCATGA T^CATGA
SacI 1340 GAGCTC GAGCT^C
SexAI 1821 ACCAGGT A^CC[AT]GGT
SgrAI 1585 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1744 CTCAAG C^T[CT][GA]AG
SspBI 755 TGTACA T^GTACA
StuI 801 AGGCCT AGG^CCT
TaqI 1320 TCGA T^CGA
Tsp509I 476 AATT ^AATT
TthHB8I 1320 TCGA T^CGA
Van91I 1886 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1878 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 315 GTAC GT^AC
756 GTAC
AflIII 752 ACGTGT A^C[GA][CT]GT
1064 ACATGT
AocI 715 CCTCAGG CC^T[ACGT]AGG
826 CCTGAGG
AviII 359 TGCGCA TGC^GCA
1562 TGCGCA
BanII 725 GGGCCC G[GA]GC[CT]^C
1340 GAGCTC
BbvCI 1152 CCTCAGC CCTCAGC(-5/-2)
4 GCTGAGG
BcnI 15 CCCGG CC^[GC]GG
1685 CCGGG
BsaWI 732 ACCGGA [AT]^CCGG[AT]
1334 ACCGGA
BsrBI 1606 GAGCGG CCGCTC(-3/-3)
1690 GAGCGG
BsrFI 182 GCCGGC [GA]^CCGG[CT]
1586 ACCGGC
BstAPI 865 GCAAAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1538 GCACTGACTGC
BstXI 1035 CCACCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1890 CCAGTGGTATGG
Bsu36I 715 CCTCAGG CC^T[ACGT]AGG
826 CCTGAGG
Cfr10I 182 GCCGGC [GA]^CCGG[CT]
1586 ACCGGC
EaeI 140 TGGCCG [CT]^GGCC[GA]
1479 CGGCCA
Eco81I 715 CCTCAGG CC^T[ACGT]AGG
826 CCTGAGG
EcoT14I 542 CCATGG C^C[AT][AT]GG
740 CCAAGG
FspI 359 TGCGCA TGC^GCA
1562 TGCGCA
HaeII 1301 AGCGCT [GA]GCGC^[CT]
1439 AGCGCC
HgaI 1272 GCGTC GACGC(5/10)
1524 GCGTC
MaeII 752 ACGT A^CGT
930 ACGT
MseI 401 TTAA T^TAA
698 TTAA
NciI 15 CCCGG CC^[GC]GG
1685 CCGGG
PvuII 237 CAGCTG CAG^CTG
271 CAGCTG
RsaI 315 GTAC GT^AC
756 GTAC
SfaNI 871 GATGC GCATC(5/9)
1676 GCATC
StyI 542 CCATGG C^C[AT][AT]GG
740 CCAAGG
Tru9I 401 TTAA T^TAA
698 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI SapI
Sau3AI Sau96I ScrFI SfcI TfiI
TseI Tsp45I TspRI XhoII