Restriction map for
pF1KB7629
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Cfr10I Cfr13I
ClaI CpoI DraII DrdI EagI
Eam1105I EclXI Eco52I EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HapII HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MluI MroI MslI MspA1I
MspI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 858 GACGCC G[GA]^CG[CT]C
AfaI 7 GTAC GT^AC
AlwNI 329 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 73 CCTGAGG CC^T[ACGT]AGG
Aor51HI 663 AGCGCT AGC^GCT
Asp700I 64 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 205 GAGCTC G[AT]GC[AT]^C
AvaI 641 CCCGAG C^[CT]CG[GA]G
BalI 687 TGGCCA TGG^CCA
BglI 718 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 654 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 654 GCTCAGC GC^T[ACGT]AGC
BsaHI 858 GACGCC G[GA]^CG[CT]C
BsiHKAI 205 GAGCTC G[AT]GC[AT]^C
BsmAI 780 GAGAC GTCTC(1/5)
BsoBI 641 CCCGAG C^[CT]CG[GA]G
BsrDI 47 GCAATG GCAATG(2/0)
Bsu36I 73 CCTGAGG CC^T[ACGT]AGG
CelII 654 GCTCAGC GC^T[ACGT]AGC
DraI 603 TTTAAA TTT^AAA
DraIII 151 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 582 CCGTGG C^C[GA][CT]GG
EaeI 687 TGGCCA [CT]^GGCC[GA]
EarI 467 CTCTTC CTCTTC(1/4)
Eco47III 663 AGCGCT AGC^GCT
Eco81I 73 CCTGAGG CC^T[ACGT]AGG
EcoNI 69 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeIII 688 GGCC GG^CC
HgaI 858 GACGC GACGC(5/10)
Hin1I 858 GACGCC G[GA]^CG[CT]C
HindIII 238 AAGCTT A^AGCTT
Ksp632I 467 CTCTTC CTCTTC(1/4)
MaeII 367 ACGT A^CGT
MfeI 500 CAATTG C^AATTG
MluNI 687 TGGCCA TGG^CCA
MscI 687 TGGCCA TGG^CCA
MunI 500 CAATTG C^AATTG
PflMI 566 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 806 GACTC GAGTC(4/5)
RsaI 7 GTAC GT^AC
SacI 205 GAGCTC GAGCT^C
SapI 466 GCTCTTC GCTCTTC(1/4)
SphI 478 GCATGC GCATG^C
SspI 903 AATATT AAT^ATT
TfiI 556 GAATC G^A[AT]TC
Van91I 566 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 64 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 702 CGCG CG^CG
825 CGCG
BanII 205 GAGCTC G[GA]GC[CT]^C
527 GAGCCC
BfaI 145 CTAG C^TAG
223 CTAG
BmyI 205 GAGCTC G[AGT]GC[ACT]^C
527 GAGCCC
BpmI 385 CTGGAG CTGGAG(16/14)
524 CTGGAG
BsiYI 69 CCTTCCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
566 CCATCCACTGG
BslI 69 CCTTCCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
566 CCATCCACTGG
Bsp1286I 205 GAGCTC G[AGT]GC[ACT]^C
527 GAGCCC
BssKI 162 CCTGG ^CC[ACGT]GG
594 CCTGG
BstNI 162 CCTGG CC^[AT]GG
594 CCTGG
BstUI 702 CGCG CG^CG
825 CGCG
Cac8I 478 GCATGC GC[ACGT]^[ACGT]GC
577 GCAAGC
EcoRI 64 GAATTC G^AATTC
538 GAATTC
EcoRII 162 CCTGG ^CC[AT]GG
594 CCTGG
HaeII 637 AGCGCC [GA]GCGC^[CT]
663 AGCGCT
HinfI 556 GAATC G^A[ACGT]TC
806 GACTC
HphI 678 GGTGA GGTGA(8/7)
777 GGTGA
MaeI 145 CTAG C^TAG
223 CTAG
MseI 514 TTAA T^TAA
604 TTAA
MvaI 162 CCTGG CC^[AT]GG
594 CCTGG
MvnI 702 CGCG CG^CG
825 CGCG
MwoI 718 GCCTGTGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
876 GCTACACTGGC
NspI 478 GCATGC [GA]CATG^[CT]
669 GCATGT
ScrFI 162 CCTGG CC^[ACGT]GG
594 CCTGG
SfaNI 772 GATGC GCATC(5/9)
708 GCATC
TaqI 536 TCGA T^CGA
908 TCGA
Tru9I 514 TTAA T^TAA
604 TTAA
Tsp45I 28 GTCAC ^GT[GC]AC
157 GTGAC
TthHB8I 536 TCGA T^CGA
908 TCGA
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbsI BbvI BpuAI BsaJI BsrI
BstF5I BstYI CfoI DdeI DpnII
Fnu4HI FokI HhaI HinP1I ItaI
MaeIII MboI MboII MflI MnlI
NdeII NlaIII NlaIV Sau3AI TseI
Tsp509I TspRI XhoII